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人卵巢上皮癌裸鼠原位移植瘤癌旁肿瘤相关基因表达及意义

Expressions and significance of tumor associated genes in tissues adjacent to human epithelial ovari an cancer of orthotopic implantation in nude mice
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摘要 目的通过对人卵巢上皮癌裸鼠原位移植瘤癌旁肿瘤相关基因表达的检测,探讨筛选癌旁正常卵巢组织的可行性。方法人卵巢上皮癌OVCAR3细胞皮下种植获取瘤源并卵巢原位移植后,取癌组织、癌旁近端组织、癌旁中段组织、癌旁远端组织及正常裸鼠卵巢组织,采用免疫组化法检测以上组织的CKG、CA125、P53、survivin、MMP-2、T1MP-2的表达量。结果40例人卵巢上皮癌裸鼠原位移植瘤模型中获取35份活检正常的癌旁残余卵巢组织,获取率87.5%(35/40)。癌组织中cK.7、CA125、P=53、suivin、MMP4及TIMP-2的表达率分别为95.O%(38/40)、95.0%(38/40)、75.0%(30/40)、85.0%(34/40)、77.5%(31/40)及77.5%(31/40);癌旁近端组织的表达率分别为71.4%(25/35)、68.6%(24/35)、57.1%(20/35)、62.9%(22/35)、60.0%(21/35)及57.1%(20/35);癌旁中段组织的表达率分别为34.3%(12/35)、31.4%(11/35)、31.4%(11/35)、31.4%(11/35)、34.3%(12/35)及31.4%(11/35);癌旁远端组织的表达率分别为25.7%(9/35)、22.9%(8/35)、25.7%(9/35)、25.7%(9/35)、28.6%(10/35)及31.4%(11/35);23份癌旁正常卵巢组织CK.7、CAl25、P53、suivin、MMP4及ⅡMP-2的表达均为阴性。免疫组化检测结果为癌旁近端组织中cK-7、CAl25、P=53、suivin、MMP-2及TIMP-2的表达低于癌组织(P〈0.05),高于癌旁中段组织及癌旁远端组织(P〈0.01);癌旁中段组织及癌旁远端组织表达差异无统计学意义(P〉0.05);cK-7、CAl25、suivin的强阳性表达率明显高于P53、MMP4、11MP-2(P〈0.01);不同病变程度获取的癌旁正常卵巢组织中的表达量差异无统计学意义(P〉0.05)。20例正常裸鼠卵巢组织CK-7、CAl25、P53、suivin、MMP4、TIMP-2的表达 Objective To investigate the feasibility in screening of normal ovarian tissues by evalu ating the expressions of tumor associated genes in tissues adjacent to human epithelial ovarian cancer of or thotopic implantation in nude mice. Methods Human epithelial ovarian cancer cell lines OVCAR3 were grown in subcutaneous tissues, and the tumor tissues were orthotopic implanted. The expressions of CK7, CA125 ,P53, survivin, MMP2, and TIMP2 were detected by immunohistochemical staining in proximal tis sues, middle tissues, distal tissues adjacent to tumor, tumor tissues, and normal ovarian tissues of nude mice. Results 35 samples ovarian tissues with normal biopsy were gained from 40 cases of human epitheli al ovarian cancers of orthotopic implantation model in nude mice. The expression rate of CK7, CA125, P53,survivin, MMP2, and TIMP2 were 95.0% (38/40), 95.0% (38/40) , 75.0% (30/40), 85.0% (34/ 40), 77.5% (31/40) , and 77.5% (31/40) in tumor tissues, respectively; 71.4% (25/35), 68.6% (24/35), 57.1% (20/35), 62. 9% (22/35), 60. 0% (21/35), and 57.1% (20/35) in proximal tissues adjacent to tumor, respectively; 34. 3% ( 12/35 ), 31.4% ( 11/35 ), 31.4% ( 11/35), 31.4% ( 11/35 ), 34. 3% ( 12/35), and 31.4% (11/35) in middle tissues adjacent to tumor, respectively; and 25.7% (9/ 35), 22.9% (8/35), 25. 7% (9/35), 25.7% (9/35), 28.6% ( 10/35 ), and 31.4% (11/35) in distal tissues adjacent to tumor, re respectively. The expressions of CK7, CA125, P53, survivin, MMP2 , and TIMP2 were all negative in 23 samples normal ovarian tissues adjacent to tumor. The expressions of CK7, CA125, P53, survivin, MMP2 and TIMP2 in proximal tissues adjacent to tumor were lower than those in tumor tissues( P 〈 0.05), and higher than those in middle tissues and in distal tissues adjacent to tumor( P 〈 0. 01 ). There were no expression difference between in middle tissues and in distal tissues ( P 〉 0. 05). The strong positive express
作者 朱根海 杨照新 王圣坦 蔡俊宏 陈春英 姚茂忠 洪澜 杨舒盈 丁莉莉 ZHU Genhai, YANG Zhaoxin,WANG Shengtan, CAI Junhong , CHEN Chunying , YA 0 Maozhong ,HONG Lan, YANG Shuying , DING Lili. Department of Gynecology, ttainan Provincial Peoples Hospital,Haikou 57031 l, China
出处 《中国医师杂志》 CAS 2012年第11期1445-1450,共6页 Journal of Chinese Physician
基金 国家自然科学基金资助项目(30960408)
关键词 卵巢肿瘤 遗传学 卵巢肿瘤 病理学 基因表达 疾病模型 动物 小鼠 肿瘤移植 Ovarian neoplasms/genetics Ovarian neoplasms/pathology Gene expression Dis-ease models, animal Mice, nude Neoplasm transplantation
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参考文献13

  • 1MacLennan AH. HRT in difficult circumstances:are there any absolute contraindications[J].Climacteric,2011,(04):409-417. 被引量:1
  • 2Kim SS. Assessment of long term endocrine function after transplantation of frozen-thawed human ovarian tissue to the heterotopic site:10 year longitudinal follow-up study[J].Journal of Assisted Reproduction and Genetics,2012,(06):489-493. 被引量:1
  • 3Terada T. Atypical polypoid adenomyoma of the uterus:an immunohistochemical study on 5 cases[J].Annals of Diagnostic Pathology,2011,(05):338-341. 被引量:1
  • 4Lin CK,Chao TK,Yu CP. The expression of six biomarkers in the four most common ovarian cancers:correlation with clinicopathological parameters[J].Acta Pathologica,Microbiologica Et Immunologica Scandinavica,2009,(03):162-175. 被引量:1
  • 5Stranahan D,Cherpelis BS,Glass LF. Immunohistochemical stains in Mohs surgery:a review[J].Dermatologic Surgery,2009,(07):1023-1034.doi:10.1111/j.1524-4725.2009.01179.x. 被引量:1
  • 6Meirow D,Hardan I,Dor J. Searching for evidence of disease and malignant cell contamination in ovarian tissue stored from hematologic cancer patients[J].Human Reproduction,2008,(05):1007-1013. 被引量:1
  • 7Heatley MK. Immunohistochemical biomarkers of value in distinguishing primary ovarian carcinoma from gastric carcinoma:a systematic review with statistical meta-analysis[J].Histopathology,2008,(03):267-276. 被引量:1
  • 8Miller K,Price JH,Dobbs SP. An immunohistochemical and morphological analysis of post-chemotherapy ovarian carcinoma[J].Journal of Clinical Pathology,2008,(05):652-657. 被引量:1
  • 9Rahma OE,Ashtar E,Czystowska. A gynecologic oncology group phase Ⅱ trial of two p53 peptide vaccine approaches:subcutaneous injection and intravenous pulsed dendritic cells in high recurrence risk ovarian cancer patients[J].Cancer Immunology Immunotherapy,2012,(03):373-384. 被引量:1
  • 10Felisiak-Golabek A,Rembiszewska A,Rzepecka IK. The Polish Ovarian Cancer Study Group (POCSG).Nuclear survivin expression is a positive prognostic factor in taxane-platinum-treated ovarian cancer patients[J].J Ovarian Res,2011,(01):20. 被引量:1
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