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鹅源草酸青霉果胶酶提高苹果出汁率工艺 预览 被引量:4

Optimization of Reaction Conditions for Pectinase Produced by Penicillium oxalicum Currie et Thom Isolated from Goose Caecum to Enhance Apple Juice Yield
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摘要 目的:以鹅源草酸青霉果胶酶为研究对象,优化鹅源草酸青霉制备的果胶酶提高苹果出汁率工艺。方法:以烟台红富士苹果为材料,以酶解温度(蜀)、酶解时间(墨)、酶添加量(X2)和初始pH值(X4)为自变量(Y),出汁率为响应值,进行Box-Behnken设计,利用DesignExpert软件进行响应面分析,建立回归模型。结果:结果表明,①利用响应面法筛选的鹅源草酸青霉果胶酶酶解苹果浆最佳条件为:温度32.91℃、酶解时间90.02min、酶添加量0.197mL/kg、pH3.96工艺条件下,苹果出汁率达到91.34%;②自变量与响应值关系的回归模型为:Y=91.72-0.4X1+0.58X2+0.60X3-0.32X4+0.42X2X3+0.50X1X4-1.75X_1^2-0.85X_2^2-1.17X_3^2-1.50X_4^2。结论:鹅源草酸青霉果胶酶能显著提高苹果出汁率;采用自变量与响应值建立的回归模型具有良好的拟合度,可以作为调控生产工艺的依据。 Objective: To optimize reaction conditions for pectinase produced by Penicillium oxalicum Currie et Thom, isolated from goose caecum, to enhance apple juice yield. Methods: Apple juice was prepared from Fuji apples from Yantai using the enzyme. A mathematical model that describes juice yield (Y) as a function of hydrolysis conditions including temperature (X1), time (X2), enzyme dosage (X3) and initial pH (X4) was established based on a Box-Behnken experimental design and subjected to response surface analysis using the Design Expert software. Results: The optimum conditions for hydrolyzing apple pulp with the microbial enzyme were found to be hydrolysis at 32.91 ℃ and initial pH 3.96 for 90.02 min with an enzyme dosage of 0.197 mL/kg. The developed mathematical model was Y=91.72-0.4X1+0.58X2+0.60X3-0.32X4+0.42X2X3+0.50X1X4-1.75X_1^2-0.85X_2^2-1.17X_3^2-1.50X_4^2. Conclusion: This enzyme can considerably enhance apple juice yield. This regression model has an excellent degree of fitting and can provide guidelines for practical production.
作者 卢燕燕 王宝维 葛文华 张名爱 岳斌 孙倩 LU Yan-yan, WANG Bao-wei, GE Wen-hua, ZHANG Ming-ai, YUE Bin, SUN Qian (1. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China; 2. Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China)
出处 《食品科学》 CAS CSCD 北大核心 2012年第24期36-41,共6页 Food Science
基金 国家水禽产业技术体系专项(CARS-43-11)
关键词 鹅源草酸青霉果胶酶 苹果出汁率 响应面法 回归模型 pectinase produced by Penicillium oxalicum Currie et Thom apple juice yield response surfacemethodology (RSM) quadratic regression model
作者简介 卢燕燕(1985-),女,硕士,研究方向为农产品加工及贮藏。E—mail:luyanyanlively@163.com. 通信作者:王宝维(1959-),男,教授,本科,研究方向为动物性食品加工及资源利用。E—mail:wangbw@qau.edu.cn
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