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转化生长因子β2对人晶状体上皮细胞E-钙黏素和α-平滑肌肌动蛋白表达的影响 预览 被引量:2

Transforming growth factor-beta 2 effects on the expression of E-cadherin and alpha-smooth muscle actin in human lens epithelial cells
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摘要 背景:有研究表明,人晶状体上皮细胞的间质转分化是后发性白内障的主要病理改变,转化生长因子β2可以诱导间质转分化的发生。目的:体外培养人晶状体上皮细胞,观察转化生长因子β2对人晶状体上皮细胞转分化相关蛋白E-钙黏蛋白和α-平滑肌肌动蛋白表达的影响。方法:利用组织块法体外培养人晶状体上皮细胞并传代,选择传5代的细胞进行实验,采用100ng/L转化生长因子β2诱导48h,反转录-聚合酶链反应方法检测E-钙黏蛋白、α-平滑肌肌动蛋白mRNA的表达,应用蛋白质印迹法Westernblot检测其蛋白表达。结果与结论:转化生长因子β2处理人晶状体上皮细胞48h后,细胞E-钙黏蛋白表达明显减弱,α-平滑肌肌动蛋白的表达明显增强。提示转化生长因子β2可以成功诱导晶状体上皮细胞间质转分化,转化生长因子β2处理的晶状体上皮细胞可以作为间质转分化的细胞模型。 BACKGROUND:Studies have shown that mesenchymal transition in human lens epithelial cells is the main pathological change after cataract.Transforming growth factor-β2 can induce the mesenchymal transition.OBJECTIVE:To in vitro culture human lens epithelial cells and to investigate the effect of transforming growth factor-β2 on the expression of E-cadherin and α-smooth muscle actin in human lens epithelial cells.METHODS:First,human lens epithelial cells were cultured in vitro by tissue block method and passaged.Then,the 5th generation of human lens epithelial cells were collected and treated with transforming growth factor-β2(100 ng/L) for inducement for 48 hours.Next,the expression of α-smooth muscle actin mRNA and E-cadherin mRNA was detected by reverse transcription-PCR,and the expression of the two proteins mentioned above were detected with Western blot method.RESULTS AND CONCLUSION:After human lens epithelial cells were treated with transforming growth factor-β2 for 48 hours,the expression of E-cadherin was decreased significantly;while the expression of α-smooth muscle actin was increased significantly.These results suggest that transforming growth factor-β2 can successfully induce mesenchymal transition in human lens epithelial cells,and the lens epithelial cells treated with transforming growth factor-β2 can be used as a cell model for mesenchymal transition.
作者 朱艳 朱玉广 钟莹莹 杜孝楠 张荣 王杰 Zhu Yan,Zhu Yu-guang,Zhong Ying-ying,Du Xiao-nan,Zhang Rong,Wang Jie Eye Center,Affiliated Hospital of Weifang Medical University,Weifang 261031,Shandong Province,China
出处 《中国组织工程研究》 CSCD 2012年第46期8636-8640,共5页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 山东省中青年科学家科研奖励基金资助项目(BS2009SW016),课题名称:整合素β1介导的MAPK通道在晶体上皮转分化中的作用及干预研究 山东省卫生厅科技计划资助项目(2009HZ105),课题名称:整合素β1在晶状体后囊混浊形成中的作用及其干预研究~~
作者简介 朱艳,女,1975年生,湖北省丹江口市人,汉族,2004年华中科技大学同济医学院毕业,硕士,讲师,主要从事青光眼、白内障方面的研究。azhu1975@SIna.com 通讯作者:朱玉广,博士,副教授,山东省潍坊医学院附属医院眼科中心,山东省潍坊市261031yg_zhu.md@tom.com
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共引文献11

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