Objective To observe the effect of Kuijieling Decoction（ KD ）on nuclear factor profilin kappa B（IκBα） gene and protein expression in Caco-2 cell model induced by interleukin-1β （IL-1β ）, and to explore its possi- ble mechanism. Methods Caco-2 cells in the growth density of 70%-80% were divided into seven groups： normal group, model group, proteasome inhibitor bortezomib group, positive drug salazosulfapyridine （SASP）group, and high-, middle- and low-dose HD groups. The cells were stimulated by IL-1β for the establishment of cell model of inflammation. Real-time quantitative PCR and Western blotting technique were used to detect gene and protein expres- sion of IκBα, respectively. Results The relative gene expression level of IκBα in the model group was lower than that in the normal group （P 〈 0.05）, and was significantly higher in the middle-dose KD group than that in the model group （P 〈 0.05）. The relative protein expression level of IκBα was lower in the model group than that in the normal group, but the difference was insignificant（P 〉 0.05）. High-dose KD group had significantly higher IκBα protein ex- pression level than the model group （P 〈 0.01 ）, Conclusion KD has an effect on the up-regulation of the gene and protein expression levels of IκBα in Caco-2 cell induced by IL-1β , and its anti-ulcerative colitis mechanism is prob- ably related to reducing the degradation of IκBα, and finally inhibiting the activation of NF-κB and relieving inflam- matory reaction.
Traditional Chinese Drug Research ＆ Clinical Pharmacology