Taxus chinensis cell line is selected by a small cell aggregate culture, and its plate culture medium is studied. The minimum size of inoculum for callus growth is 14.5?g (cell dry weigh)/L medium, and the growth cycle of callus is about 35 days. Improved MS Medium is suitable for callus growth. The cell growth rate can be promoted by increasing the glucose concentration to 5?g·L -1 , sucrose to 30?g·L -1 , and also by supplementing with yeast extract, glycine, Vitamin C, folic acid, choline chlorice and calcium pantothenate. The taxol content can also be promoted by the above treatments.
Journal of Huazhong University of Science and Technology
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Taxus chinensis cell
small cell aggregate culture