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人淋巴细胞微核率辐射剂量效应曲线的研究 预览 被引量:2

The study of radiation dose-effect curves with micronuclei in human lymphocytes
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摘要 目的 用^60Coγ射线照射人离体血,建立人淋巴细胞微核剂量效应曲线。方法 4名健康男性外周血样以^60Coγ射线(0.1、0.25、0.5、1.0、2.0、3.0、4.0、5.0Gy,剂量率0.5Gy/min)照射后,用胞质阻断微核法(CB)和常规微核法建立剂量效应曲线。结果 在0~5.0Gy剂量范围内,两种方法微核率随剂量增加而增加,呈明确的剂量效应关系。曲线拟合表明,回归方程式成立,拟合度良 Objective To establish the relationship curves between radiation dose and micronucleus frequencies in human lymphocytes.Methods 4 healthy human blood samples were irradiated with various doses (0.1,0.25, 0.5, 1.0, 2.0, 3.0, 4.0 and 5 0 Gy) of 60 Co γ ray at 37℃(dose rate 0.5 Gy/min) and prepared by using cytokinesis block micronucleus method and conventional method in vitro.Results Dose dependent increase of micronuclei rate were observed within the dose range of 0~5.0 Gy under two methods. The curve regressions were established with high correlation ( R 2 closing to 1, P <0.01).Conclusion The curves established by this study can be used as biological dosimeters to estimate the exposure dose in a radiation accident.
作者 梁丽燕 郑巧玲 LIANG Li yan, ZHENG Qiao ling,HUANG Jian xun, et al (Guangdong Institute of Occupational Medicine, Guangzhou 510300, China)
出处 《中国职业医学》 CAS 2000年第4期 4-6,共3页 China Occupational Medicine
基金 广东省医学科学研究基金!(960 4 )
关键词 微核 剂量效应曲线 离体血照射 淋巴细胞微核率 micronucleus, dose effect curves, radiation
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