The preparation process for tilapia scale collagen peptide calcium chelate was optimized using response surface methodology,and a mathematical model was established to describe peptide calcium chelate ratio as a function of time,mass ratio of peptide to calcium chloride,temperature and pH. The optimal preparation parameters obtained were as follows..time 39min,mass ratio of peptide to calcium chloride 3.3：1, temperature 61℃ and pH7.2. Under these conditions,the experimental value was 65.01% ,which was no significant difference with the predicted value （65.05%）. Infrared spectroscopy showed that tilapia scale collagen peptide calcium chelate was formed. These experimental results provided a theoretical basis for the development of new calcium preparations and the high-value utilization of fish scales.
Science and Technology of Food Industry
response surface methodology fish scale collagen peptide calcium chelate