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炎症性肠病患者肠道菌群结构的变化及其与炎性指标的关系 预览 被引量:30

Changes of fecal flora and its correlation with inflammatory indicators in patients with inflammatory bowel disease
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摘要 目的探讨炎症性肠病患者肠道菌群结构变化及其与发病的关系。方法收集167例炎症性肠病患者[其中溃疡性结肠炎(UC)113例,克罗恩病(CD)54例]、54例健康志愿者新鲜粪便,用梯度稀释法定量培养进行菌群分析,同时收集白细胞、血小板、C反应蛋白、血沉四项炎性指标数据评价其与菌群变化的相关性。结果UC患者与健康对照组相比,肠球菌(6.60±0.23,P〈0.01)、酵母菌(2.22±0.27,P〈0.05)、拟杆菌(5.57±0.28,P〈0.001)、双歧杆菌(5.08±0.30,P〈0.01)、消化球菌(6.22±0.25,P〈0.001)、乳酸杆菌(6.00±0.26,P〈0.001)、小梭菌(3.57±0.30,P〈0.05)的数量显著增加,真杆菌(1.56±0.24,P〈0.01)的数量显著下降。CD患者与健康对照组相比,肠球菌(6.93±0.28,P〈0.01)、舀爹母菌(2.73±0.37,P〈0.01)、拟杆菌(4.32±0.52,P〈0.05)、双歧杆菌(4.88±O.42,P〈0.05)、消化球菌(6.19±0.32,P〈0.01)、乳酸杆菌(4.73±0.47,P〈0.001)的数量显著增加,真杆菌(1.01±0.29,P〈0.01)、小梭菌(0.87±0.31,P〈0.01)的数量显著下降。培养附眭率与其数量变化结果基本符合。炎性指标与菌群相关不大。结论与健康对照组相比,IBD患者肠道菌群呈失衡状态。真杆菌减少与炎症性肠病相关。 Objective To investigate the changes in fecal flora and its correlation with the occurrence and progression of inflammatory bowel disease (IBD). Methods We collected fresh fecal specimens from 167 IBD patients (including 113 with ulcerative colitis and 54 with Crohn's disease) and 54 healthy volunteers. The fecal flora was analyzed by gradient dilution method and the data of inflammatory markers including WBC, PLT, CRP and ESR were collected to assess the association between the fecal flora and the inflammatory markers. Results The species Enterrococcus (6.60±0.23, P〈0.01), Saccharomyces (2.22 ± 0.27, P〈0.05), Bacteriodes (5.57 ± 0.28, P〈0.001), Bifidobacterium (5.08 ± 0.30, P〈0.01), Peptococcus (6.22 ± 0.25, P〈0.001), Lactobacillus (6.00±0.26, P〈0.001), and Clostridium (3.57±0.30, P〈0.05) all increased significantly, while Eubacterium (1.56±0.24, P〈 0.01) reduced markedly in patients with ulcerative colitis compared with those in the control subjects. Enterrococcus (6.93±0.28, P〈0.01), Saccharomyces (2.73±0.37, P〈0.01), Bacteriodes (4.32±0.52, P〈0.05), BiJidobacterium (4.88±0.42, P〈0.05), Peptococcus (6.19± 0.32, P〈0.01) and Lactobacillus (4.73±0.47, P〈0.001) all increased significantly and Eubacterium (1.01±0.29, P〈0.01) and Clostridium (0.87±0.31, P〈0.01) decreased in patients with Crohn's disease. The positivity rates of bacterial culture were consistent with the results of quantitative analysis of the fecal flora. The changes in fecal flora did not show a significant correlation with these inflammatory markers. Conclusion IBD patients have fecal flora imbalance compared with the healthy controls, and this imbalance may contribute to the occurrence and progression of IBD. The decline of Eubacterium contributes to the occurrence and development of IBD.
作者 张婷 陈烨 王中秋 周有连 张绍衡 王浦 谢珊 姜泊 ZHANG Ting, CHEN Ye, WANG Zhongqiu, ZHOU Youlian, ZHANG Shaoheng, WANG Pu, XIE Shan, JIANG Bo Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangdong Provincial Key Laboratory of Gastroenterology, Guangzhou 510515, China
出处 《南方医科大学学报》 CAS CSCD 北大核心 2013年第10期1474-1477,1498共5页 Journal of Southern Medical University
基金 国家自然科学基金(81070291)
关键词 炎症性肠病 肠道菌群 炎症指标 Inflammatory bowel disease intestinal flora inflammatory markers
作者简介 张婷,硕士,E-mail:zhangtingyikeda@126.com; 陈烨,医学博士,教授,博士生导师,电话:020-61641543,E-mail:chenye2001@hotmail.com。 通信作者:姜泊,医学博士,教授,博士生导师,电话:020-61641541,E-mail:drjiang@163.com
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