期刊文献+

荧光偏振法测定红细胞膜粘度在药学方面的应用 预览

APPLICATION OF DETERMINATION OF THE FLUIDITY OF ERYTHROCYTE MEMBRANCE BY FLUORESCENCE POLARIZATION METHOD TO THE AREA OF PHARMACOLOGICAL RESEARCH
在线阅读 下载PDF
收藏 分享 导出
摘要 本文介绍荧光偏振法应用于“血瘀”的动物模型,观察用人参后血液红细胞膜粘度的变化,借以研究人参对于“血瘀”用药的药效作用. Cytomembrane damage, cell senility and the aging can change the fluidity of biomembrances. The viscosity of cell membrance is increased.In order to study changes of viscosity or fluidity of the cell membrance, we used rat erythrocytes as the source of membrance.Erythrocyte was extracted by Michael's method. After hemolysis the red cell membrancec were washed with buffer and collected by highspeed centrifugation.All cytomembrances were labelled with DPH(1,6-dipheny-1,3, 5-hexatriene, 2×10-6M, 4ml)at 25℃, for 30 minutes and fluorescence polarization was determined with PERKIN-ELMER MPF-66.The results were compared with animal plasma and blood viscosity. We used this fluorescence polarization determination method to study the pharmacological effects of Ginseng on preventing senility and treating arteriosclerosis. The results demonstrate that Ginseng can increase the fluidity(or decrease the viscosity)of red cell membrarce significantly. Ginseng can increase blood flow rates of artificial thrombosis.The experiment fully proved that the method is a practical and effective measure for the researches of pharaceutics.
作者 杨世杰 孙(九虎) 张家颖 赵风章 麦荫乔 孙红 杨晓静 张泓嵬 张志伟 张树臣 Yang Shijie Sun Xiao Mai Yinqiao(Central Laboratory of Molecular Biology, Norman Bethune Universityof Medicine Scineces)Sun Hong Yang Xiaojing Zhang Hongwei Zhang Zhiwei Zhng Shuchen(Institute of Traditional Chinese Medicine and Herbs in Jilin province)
出处 《发光学报》 EI CAS CSCD 北大核心 1991年第1期 57-60,共4页 Chinese Journal of Luminescence
  • 相关文献

参考文献2

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈