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四妙勇安汤合生脉饮对缺氧/复氧乳鼠心肌细胞凋亡的影响 预览 被引量:1

Effect of Simiaoyongan Decoction with Shengmai Decoction on Apoptosis of Hypoxia/ Reoxygenation Neonatal Rat Cardiomyocytes
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摘要 目的观察四妙勇安汤合生脉饮对缺氧/复氧乳鼠心肌细胞细胞凋亡的影响,探讨其心肌细胞保护作用。方法采用胰蛋白和胶原酶联合消化的方法原代培养的SD乳鼠心肌细胞,利用高纯氮气建立心肌细胞缺氧/复氧模型。实验分为正常组、模型组和药物干预组(予四妙勇安汤合生脉饮)。采用流式细胞术AnnexinV-FITC/PI双染法检测心肌细胞凋亡率。结果与正常组比较,模型组及药物干预组心肌细胞凋亡率均升高(P〈0.05)。与模型组比较,药物干预组心肌细胞凋亡率下降(P〈0.05)。结论四妙勇安汤合生脉饮对缺氧/复氧后心肌细胞凋亡有保护作用。 Objective To observe the effect of Simiaoyongan decoction with Shengmai decoction on apoptosis of hypoxia/reoxygenation model of primary cultred neonatal rat cardiomyocytes. Methods The combination of trypsin and collagenase was used to isolate neonatal rat myocardial cells. Hypoxia/ reoxygenation model of cardiomyocytes was made by high purity nitrogen gas. Primary cultured neonatal rat cardiomyocytes were randomly divided into control group, model group and treatment group (treated with Simiaoyongan decoction and Shengmai decoction). The myocardial apoptotic rates were detected by Annexin V-FITC/PI double-staining method. Results Compared with the control group, the apoptotic rate of model group and treatment group was high (P〈0.05). Drug intervention lowered the apoptotic rate compared with the model group, the difference was significant (P 〈 0.05). Conclusion The alcohol sedimentation solution of Simiaoyongan decoction with Shengmai decoction played a protective role on hypoxia/reoxygenation cardiomyocytes.
作者 于慧卿 孟云辉 李永新 王强 刘真 刘晓艳 李武卫 YU Hui-qing, MENG Yun-hui, LI Yong-xin, WANG Qiang, LIU Zhen, LIU Xiao-yan, LI Wu-wei (Shijiazhuang City Hospital ofTCM, Shijiazhuang 050051, China)
机构地区 石家庄市中医院
出处 《中国中医药信息杂志》 CAS CSCD 2013年第12期35-37,共3页 Chinese Journal of Information on Traditional Chinese Medicine
基金 河北省中医药管理局课题(2011053)
关键词 四妙勇安汤 生脉饮 心肌细胞凋亡 大鼠 Simiaoyongan decoction Shengmai decoction: apoptosis of myocardial cells: rats
作者简介 通讯作者:刘真,E-mail:1iuzhen580527@126.com
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