Objective： To investigate the effect of adenovirus mediated interleukin-10 gene on streptozotocin （STZ）-induced type 1 diabetic mellitus （T1DM） in SD rats, and to investigate the expression of Bcl-2 and Bax. Methods： Forty male SD mice aged from four to five weeks were randomly divided into 4 groups. Group 1 （vacuity group） was without special disposal; Group 2 was diabetes mellitus control goup; Group 3 received the intraperitoneal injection of adenovirus vector mediated empty green fluorescence protein （Ad-eGFP）; Group 4 received intraperitoneal injection ofadenovirus vector mediated IL-10 gene （0.2 mL）. Group2, 3 and 4 received intraperitoneal injection of stz 50 mg/kg in order to induce diabetic mellitus of SD mice. Pancreatic histology was measured for determination of insulitis grades. Apoptosis was measured by using a TUNEL assay. Expressions of Bcl-2 and Bax were estimated by immunohistochemistry analysis. Results： Compared with that in the control group, Ad-mlL-10 group had lower incidence in diabetes and lower degree of average blood glucose; Pancrea pathology showed that inflammatory infiltration was lighter and beta-cell apoptosis decreased. Decreased expression of Bax and incresed rate of Bcl-2/Bax was observed in the Ad-mlL-10 group. Conclusions： IL-10 gene therapie is able to decreasethe average blood glucose and exhibits anti-apoptotic ability. The expressions of Bax and Bcl-2 in the pancreas showed positive correlation with apoptosis.
Progress in Modern Biomedicine