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IL-10基因对糖尿病大鼠胰岛β细胞凋亡的保护作用 被引量:2

The Protective Effect of IL-10 Gene on Pancreatic Beta-cell in STZ-induced Diabetic Rats
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摘要 目的:研究白细胞介素10(IL-10)基因对链脲佐菌素(STZ)诱导的糖尿病大鼠胰腺炎症浸润程度及胰腺组织中Bcl-2及Bax表达的影响。方法:建立链脲佐茵素性糖尿病模型,腺病毒介导的IL-10基因(Ad-mIL-10)腹腔注射。检测大鼠空腹血糖值;免疫组织化学法观察胰腺炎症浸润程度;TUNEL法检测胰岛细胞凋亡;免疫组化方法观察Ad-mIL-10对实验性糖尿病大鼠胰岛凋亡调控基因Bax和Bcl.2表达的影响。结果:Ad-mlL-10腹腔注射糖尿病发病率低,平均血糖水平低,可以降低胰腺炎症浸润程度,减少胰岛细胞凋亡。给予Ad-mlL-10后大鼠Bax基因的表达明显下降,Bcl-2与Bax的比值明显增加。结论:IL-10基因对实验性糖尿病大鼠有降血糖作用,减少胰岛细胞凋亡,与调节Bcl-2与Bax基因的表达有关。 Objective: To investigate the effect of adenovirus mediated interleukin-10 gene on streptozotocin (STZ)-induced type 1 diabetic mellitus (T1DM) in SD rats, and to investigate the expression of Bcl-2 and Bax. Methods: Forty male SD mice aged from four to five weeks were randomly divided into 4 groups. Group 1 (vacuity group) was without special disposal; Group 2 was diabetes mellitus control goup; Group 3 received the intraperitoneal injection of adenovirus vector mediated empty green fluorescence protein (Ad-eGFP); Group 4 received intraperitoneal injection ofadenovirus vector mediated IL-10 gene (0.2 mL). Group2, 3 and 4 received intraperitoneal injection of stz 50 mg/kg in order to induce diabetic mellitus of SD mice. Pancreatic histology was measured for determination of insulitis grades. Apoptosis was measured by using a TUNEL assay. Expressions of Bcl-2 and Bax were estimated by immunohistochemistry analysis. Results: Compared with that in the control group, Ad-mlL-10 group had lower incidence in diabetes and lower degree of average blood glucose; Pancrea pathology showed that inflammatory infiltration was lighter and beta-cell apoptosis decreased. Decreased expression of Bax and incresed rate of Bcl-2/Bax was observed in the Ad-mlL-10 group. Conclusions: IL-10 gene therapie is able to decreasethe average blood glucose and exhibits anti-apoptotic ability. The expressions of Bax and Bcl-2 in the pancreas showed positive correlation with apoptosis.
作者 徐爱晶 陈志红 田飞 阎丽华 朱伟 XU Ai-jing, CHEN Zhi-hong, TIAN Fei, YAN Li-hua, ZHU Wei(1 Department of Pediatric Endocrinology and Membolism, Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong, 510623, China; 2 Department of Pediatrics, the Afliliated Hospital of Qingdao Medical College, Qingdao University, Qingdao, Shandong, 266003, China; 3 Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan, Guangdong, 523808, China)
出处 《现代生物医学进展》 CAS 2014年第4期671-675,共5页 Progress in Modern Biomedicine
基金 广东省自然科学基金项目(s2012040006330) 广东省科技计划项目(20128031800016)
关键词 腺病毒 IL-10 胰岛细胞 凋亡 Adenovirus IL-10 Islet beta cells Apoptosis
作者简介 徐爱晶,女,博士,主治医师,研究方向:内分泌及遗传代谢性疾病,电话:020-38076127,E-mail:xuaj246@126.com 通讯作者:朱伟,硕士生导师,副教授,E-mail:zhuweil68@yahoo.cn
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参考文献20

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