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缬沙坦治疗原发性高血压患者血浆蛋白质组研究 预览 被引量:3

Plasma proteomic level in hypertensive patients before and after valsartan treatment
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摘要 目的探讨缬沙坦治疗前后原发性高血压(EH)患者血浆蛋白质组差异。方法筛选健康正常血压者及EH患者,设为正常对照组(NT组)、EH患者(EH组)、缬沙坦治疗伴有心肌肥厚EH患者(EHT1组)和缬沙坦治疗不伴有心肌肥厚EH患者(EHT2组),每组21例。分离血浆,去除高丰度蛋白质,制备蛋白质样品,使用二雏电泳结合基质辅助激光解析电离飞行时间质谱技术,进行各组血浆蛋白质组的分析和鉴定。结果与NT组比较,EH组和EHT1组的血肌酐显著升高(P〈0.05),EH组、EHT1组和EHT2组收缩压显著升高(P〈0.05)。与NT组比较,EH组血浆中存在22个差异表达的蛋白质点,其中16个表达量上调,6个下调;EHT1纽存在12个,其中10个上调,2个下调;EHT2组存在11个,9个上调,2个下调。与EH组比较,EHT1存在17个差异表达的蛋白质点,其中4个上调,13个下调;EHT2组存在19个,其中5个上调,15个下调(P〈0.05)。结论EH患者血浆蛋白质组与正常血压者之间存在明显差异,缬沙坦治疗可以缓解但并不能完全恢复血浆蛋白质组的水平。 Objective To study the different plasma proteomic levels in essential hypertension (EH) patients before and after valsartan treatment. Methods Twenty-one healthy normotensive subjects served as a normal control group, 21 untreated EH patients served as an EH group, 21 valsartan-treated EH patients with myocardial hypertrophy served as an EHT1 group and 21 val- sartan-treated EH patients without myocardial hypertrophy served as an EHT2 group in this study. Protein samples were prepared by isolating plasm with high abundant protein removed. The plasm proteomic levels in different groups were analyzed by 2-dimensional electrophoresis in com- bination with matrix-aided laser disaaociation spectrophy and identified by Western blot. Results The plasma creatinine level was significantly higher in EH group and EHT1 group than in normal control group (P〈0. 05). The systolic blood pressure was significantly higher in EH group, EHT1 group and EHT2 group than in normal control group (P〈0.05). The ST,PWT and LVMI were much better in EHT2 group than in EHT1 group(P〈0.05). The expression levels were higher in 16,10,9 protein spots and lower in 6,2,2 protein spots in EH group and EHT1 group than in normal control group,the expression level was lower in 6 protein spots in EHT1 group, higher in 1 protein spot and lower in 7 protein spots in EHT1 group than in EH group (P〈 0.05). Conclusion The plasma proteomic level is significantly different in EH patients and nor- motensive subjects,which can be relieved but not completely recovered by valsartan.
作者 王佐广 彭晓云 李梅 张蓓 王丽娟 刘洁琳 刘雅 魏永祥 温绍君 WANG Zuo-guang,PENG Xiao-yun, LI Mei, et al (Beijing Institute of Heart ,Lung,Blood Vessel Diseases ,Affiliated Beijing Anzhen Hospital of Capital Medical University, Beijing 100029, China)
出处 《中华老年心脑血管病杂志》 CAS 北大核心 2014年第4期343-348,共6页 Chinese Journal of Geriatric Cardiovascular and Cerebrovascular Diseases
基金 国家自然科学基金(81370229,81270216) 北京市自然科学基金(7102045,7133232,7120001)
关键词 高血压 蛋白质组 抗高血压药 心室重构 hypertension proteome antihypertensive agents ventricular remodeling
作者简介 通信作者:温绍君,E-mail:wenshaojun@aliyun.com.cn
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参考文献25

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