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溃结灵含药血清对大鼠结肠上皮细胞损伤模型MUC-2及TGF-α含量的影响

Effects of Serum Containing Kuijieling on the Contents of MUC-2 and TGF-α in Rat Colonic Epithelial Cell Damage Model
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摘要 目的:观察溃结灵含药血清对结肠上皮细胞损伤模型MUC-2及TGF-α蛋白含量的影响,进一步探讨渍结灵促进肠黏膜损伤修复的机理。方法:TNF-α(50ng/mL)干预大鼠结肠上皮细胞6h造成细胞损伤模型,采用Elisa方法检测不同浓度溃结灵含药血清对细胞损伤模型MUC-2及TGF-α蛋白含量的影响。结果:①结肠上皮细胞损伤模型组MUC-2蛋白含量较空白对照组升高(P〈0.05);②溃结灵含药血清高剂量组MUC-2蛋白含量较模型对照组升高(P〈0.05);溃结灵含药血清高剂量组TGF-α蛋白含量较模型对照组升高(P〈0.01)。结论:溃结灵含药血清可促进TNF-α致结肠上皮细胞损伤模型中MUC-2、TGF-α含量升高,进而促进受损肠黏膜上皮的修复。 Objective To establish the damage model of rat colonic epithelial cell, and observe the effect of serum containing Kuijieling on contents of MUC-2 and TGF-α in the cell damage model in order to explore the mechanism of Kuijieling promoting to repair the injury of mucous membrane. Methods The colonic epithelial cell was intervened with 50ng/mL TNF-α for 6h to induce the cell damage model. The contents of MUC-2 and TGF-α were detected by using Elisakit. Results (1)Compared with the blank control group, the MUC-2 content in the cell damage model group was obviously increased(P〈0.05).(2)The contents of MUC-2 and TGF-α in high- dosage group (10% Kuijielin drug-contained serum) were significantly higher than that in the model group (P〈0.05, P〈0.01). Conclusion Kuijieling drug-contained serum could boost the contents of MUC-2 and TNF-α in rat colonic epithelial cell damage model, which might promote to repair of the impaired colonic mucous membrane epithelium.
作者 祁燕 李燕舞 王汝俊 巫燕莉 QI Yan, LI Yan-wu,WANG Ru-jun, WU Yan-li (Institute of Spleen and Stomach, Guangzhou University of Chinese Medicine, Guangdong Guangzhou 510405)
出处 《深圳中西医结合杂志》 2014年第1期1-4,共4页 Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
基金 国家自然科学基金资助项目(30772754)
关键词 含药血清 结肠上皮细胞 MUC-2 TGF-Α Kuijieling Drug-contained serum Colonic epithelial cell MUC-2 TGF-α
作者简介 [通讯作者]李燕舞(E-mail:lyw_7614@126.com)
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