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不同时间电针对血管性痴呆小鼠行为学及海马单胺类神经递质的影响 被引量:16

Effect of Electroacupuncture Intervention at Different Time-points of Post-modeling on Hip- pocampal Monoamine Neurotransmitter Levels in Mice with Vascular Dementia
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摘要 目的:观察不同时间电针对血管性痴呆(VD)小鼠学习记忆能力及海马去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)含量的影响,探讨电针改善VD学习记忆功能障碍的可能作用机制。方法:60只昆明小鼠随机分为两批,每批各设假手术组、模型组、电针组,每组10只。采用颈总动脉结扎缺血再灌注的方法复制VD模型。第1批于造模后苏醒当日开始电针干预,第2批于造模第3天开始进行电针干预。电针组针刺“百会”“大椎”及双侧“足三里”“膈俞”,每次10min,治疗15d。观察各组小鼠跳台学习成绩及跳台记忆成绩,荧光分光光度法检测海马NE、DA、5-HT的含量变化。结果:与假手术组比较,模型组小鼠的学习和记忆成绩均明显下降(P〈0.01),海马NE、DA、5-HT含量均显著降低(P〈0.01);与模型组比较,电针组小鼠学习和记忆成绩均明显提高(P〈0.01),海马NE、DA、5-HT含量均显著升高(P〈0.01),且造模第3天电针组优于造模当天电针组(P〈0.05,P〈0.01)。结论:电针能够改善VD学习记忆功能障碍,其作用机制与改善海马中单胺类神经递质代谢紊乱有关,并初步发现介入干预时间不同,其治疗效应存在差异。 Objective To observe the effect of electrocupuncture (EA) intervention at different time-points of post-mo- deling on behavior and hippocampal monoamine neurotransmitter noradrenalin (NE), dopamine (DA) and 5-hydroxytrypamine (5-HT) contents in vascular dementia (VD) mice, so as to study its mechanism underlying improvement of VD. Methods A to- tal of 60 Kunming mice were randomized into sham-operation control (n = 20), VD model (n = 20), EA-day (D)-I (EA treatment was given from the 1st day on after modeling, n = 10), EA-D-3 (EA was given from the 3rd day on after modeling, n = 10) groups. VD model was established by occlusion of the bilateral cervical common arteries and reperfusion. EA (2 Hz/80 Hz) was applied to "Baihui" (GV 20), "Dazhui" (GV 14) ,"Zusanli" (ST 36) and "Geshu'(BL 17) for 10 min, once daily for 15 days. Hippocampal NE, DA and 5-HT contents were assayed by fluorospectrophotometry. The mouse's learning-memory ability was assessed by step-down tests. Results In comparison with the sham-operation control group, the learning-memory ability (marked increase of reaction time and error times, decrease of step-down latency) was apparently lowered in the model group (P〈0.01). The hippocampal NE, DA and 5-HT contents were significantly lower in the model group than in the sham-operation group (P〈0.01). Compared with the model group, the mice's learning-memory ability (marked decrease of reaction time and error times, increase of step-down latency) was significantly increased in EA intervention groups ( P〈0. 01 ), and hippocampal NE, DA and 5-HT levels were significantly increased ( P〈0.01 ), and the effect of EA-D-3 group was obviously better than that of the EA-Dol group (P〈0.05, P〈0.01). Conclusion EA can improve the VD mice's learning-memory ability, which is closely related to its effects in up-regulating hippocampal NE, DA and 5-HT contents, and the effect of later EA interventi
作者 张会珍 梁玉磊 张雪静 张闯 孙彦辉 张秀景 许晓康 贾睿帼 王鑫国 ZHANG Hui-zhen, LIANG Yu lei, ZHANG Xue-jing, ZHANG Chuang, SUN Yan-hui, ZHANG Xiu-jing, XU Xiao-kang, JIA Rui guo , WANG Xin-guo ( Hebei College of Chinese Medicine, Shijiazhuang 050091 , China; 1 Hebei Medical University, Shij iazhuang 050017)
出处 《针刺研究》 CAS CSCD 北大核心 2014年第2期142-147,共6页 Acupuncture Research
基金 河北省科技厅科技攻关项目(No.042761388) 河北省中医药管理局资助项目(No.20040i0、No.2006058)
关键词 电针 血管疾病 学习记忆能力 去甲肾上腺素 多巴胺 5-羟色胺 海马 Electrocupuncture Vascular disease Learning-memory ability Noradrenalin Dopamine 5-hydroxytrypamine~ Hippocampus
作者简介 张会珍(1962-),女,教授,研究方向:针灸防治脑血管病。Email:zhz6212202@163.com 通信作者:王鑫国(1966-),男,教授,研究方向:中医防治老年痴呆。Email:wangxingguozy@163.com.
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