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孕妇血浆循环DNA的检测与动态分析 被引量:2

Study of the quantitative change of circulating DNA in matemal plasma.
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摘要 目的本文旨在探索使用荧光定量PCR方法对孕妇全血和血浆中DNA含量进行动态分析,从而为利用孕母血浆循环DNA进行无创产前诊断奠定基础。方法?提取60例早、中、晚孕期孕妇全血基因组DNA和血浆循环DNA.应用实时荧光定量聚合酶链式反应(FQ—PCR)技术测定不同孕期的孕妇全血和血浆中保守基因(β-actin)的含量,比较不同孕期孕妇全血和血浆DNA含量的变化。结果(1)早、中、晚孕期孕妇全血DNA含量ct平均值为18.44±0.56828、18.35±0.93394、18.15±0.57410,3组比较没有显著性差异(P〉0.05);(2)孕妇早、中、晚期孕组血浆DNA含量ct平均值25.59±1.17673、25.50±1.59892、24.35±2.24301,早、中期孕组血浆DNA没有显著性差异(P〉0.05),早、晚期和中、晚期孕组血浆DNA在两者之间有显著性差异(P〈0.05);(3)排除全血DNA干扰,早、中、晚孕期孕妇血浆中DNA相时定量值为0.007401、0.007401、0.013602。结论用实时荧光定量PCR的方法可以检测到孕妇血浆中循环DNA的含量变化,随孕周的增加而逐渐增加,可以利用其进行无创产前诊断。 Objective: To evaluate the quantitative change of circulating DNA in maternal plasma by real - time fluorescent quanti- tative polymerase chain reaction ( FQ - PCR), so as to lay the foundation for non - invasive prenatal diagnosis. Methods: Whole blood DNA and plasma DNA was extracted from sixty eases of early, middle and late pregnancy women respectively. FQ - PCR was used to determine β - actin gene concentration in different stage of pregnancy, whole blood and plasma DNA levels were compared in three different pregnancy stage groups. Results : ( 1 ) The mean Ct values of whole blood DNA in the early, middle and late pregnancy groups were 18.44 ± 0. 56828, 18. 35 ± O. 93394, 18. 15 ± O. 57410, there is no significant difference between the three groups (P 〉 0. 05). (2) The mean Ct values of plasma DNA in the three groups were 25. 59 ± 1. 17673, 25.50 ± 1. 59892, 24. 35 ± 2. 24301, with significant difference between the early and late group (P 〈 0. 05 ) and with significant difference between the middle and late group (P 〈 O. 05 ). (3) The relative quantitative values of circulating DNA in early, middle and late groups were 0. 007401, 0. 007401, O. 013602. Conclusion: The circulating DNA in maternal plasma could be detected by FQ -PCR and it increased with ges- tational aging, which may provide a theoretical basis for developing non - invasive prenatal diagnostic methods.
作者 冉贵萍 吴卫华 谢艳平 张圣芳 方文 RAN Gui -ping, WU Wei - hua, XIE Yah - ping, ZHANG Sheng -fang, FANG Wen. ( 1. Shanghai Fengcheng Hospital, Shanghai, 201411, China; 2. Guiyang Medical College Affiliated Hospital, Guiyang , 550004, China)
出处 《中国优生与遗传杂志》 2014年第4期6-7,40共3页 Chinese Journal of Birth Health & Heredity
关键词 孕妇血浆 循环DNA β—actin Maternal plasma Circulating DNA β - actin
作者简介 通讯作者:冉贵萍
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