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添加氧载体对γ-多聚谷氨酸发酵的影响 预览

Effect on Poly-γ-Glutamic Acid Fermentation by Addition of Oxygen-Vectors
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摘要 针对γ-多聚谷氨酸(Poly-γ-glutamic acid,γ-PGA)好氧发酵过程中存在的供氧不足而导致产量偏低的问题,探究了不同氧载体对γ-PGA发酵的影响,并对最适氧载体的添加量和添加时间进行了优化。结果表明,正十六烷为最适氧载体,其最佳添加条件为:添加量为0.5%(体积比),添加时间为0h,发酵48h,γ-PGA产量达到最高值为(18.62±0.84)mg/mL,相比对照组提高了30%以上。本研究为通过添加氧载体,增加溶氧来提高γ-PGA发酵产量提供了理论依据。 In order to solve the problem of low yield caused by insufficient oxygen supply during the aerobic fermentation of poly-γ-glutamic acid (γ-PGA ).The effects of different oxygen-vectors on γ-PGA fermentation was studied.The addition amount and time of the most effective oxygen-vector were optimized as well.The results showed that n-hexadecane was the most effective oxygen-vector.The optimal addition condition was 0.5%(V:V )and the adding time was 0h.After 48h fermentation,the yield of γ-PGA was increased to (18.62±0.84)mg/mL,which increased more than 30% compared with the control group.This study provided a theoretical basis for increasing the yield of γ-PGA by addition of oxygen -vectors to increase dissolved oxygen.
作者 冯文心 袁志怡 常忠义 高红亮 贾彩凤 FENG Wen-xin;YUAN Zhi-yi;CHANG Zhong-yi;GAO Hong-liang;JIA Cai-feng(School of Life Science,East China Normal University,Shanghai 200241,China)
出处 《食品工业科技》 CAS 北大核心 2019年第1期127-130,142共5页 Science and Technology of Food Industry
关键词 γ-多聚谷氨酸 正十六烷 氧载体 发酵 poly-γ,-glutamic acid (γ-PGA) n-hexadecane oxygen-vectors fermentation
作者简介 冯文心(1997-),女,本科,研究方向:生物技术,E-mail:whalefengwx@163.com;通讯作者:贾彩凤(1979-),女,博士,高级实验师,研究方向:食品微生物学,E-mail:cfjia@bio.ecnu.edu.cn。
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