Aiming to determine the effects of four cultivation methods including transplantation, potash fertilizer,pruning, and ethylene on the protective enzymes, PAL activity and MDA content of Dalbergia odorifera, young Dalbergia odorifera 6~8 cm in diameter was selected as the study object. The change regulation of SOD, POD,CAT, PAL activity and MDA content in Dalbergia odorifera were studied with different potash dosage, pruning strength, percentage of ethylene and mechanical treatment during transplantation. The results showed that the four cultivation methods which were transplantation, potash fertilizer, pruning, and ethylene, all had varying degrees of effects on protective enzymes, PAL activity and MDA content in leaves of Dalbergia odorifera. The activities of four enzymes increased in the order of full crown shift>root break>crown removal>crown shift>CK. There was a significant positive correlation between pruning intensity and potassium fertilizer dosage, and they increased first and then decreased with increasing ethylene percentage. The increase in MDA content was negatively correlated with the pruning intensity and the amount of potash fertilizer, with the ethylene percentage increased, it dropped first and then rises;in the transplantation experiment, the order of CK >de-crown shift >crown removal >root removal>full crown shift was sequentially increased.
Molecular Plant Breeding