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酸枣仁中化学成分的UHPLC-LTQ-Orbitrap-MS快速分析

Rapid analysis of chemical components in Zizyphi Spinosae Semen by UHPLC-LTQ-Orbitrap-MS
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摘要 基于UHPLC-LTQ-Orbitrap-MS技术对酸枣仁中化学成分进行定性分析。采用Waters Acquity UPLC BEH-C18色谱柱(2. 1 mm×50 mm,1. 7μm),以乙腈(A)-0. 1%甲酸水溶液(B)为流动相梯度洗脱,体积流量0. 3 m L·min^-1;在电喷雾负离子模式下采集数据。将母离子列表(parent ion list,PIL)-MS2和高能碰撞(high energy collision dissociation,HCD)技术,用于酸枣仁中三萜皂苷成分的鉴别。结合质谱数据和文献数据,共鉴定、推断出24个化合物,4个未知化合物;其中包括21个黄酮类化合物,5个皂苷类化合物,2个三萜类化合物。采用UHPLC-LTQ-Orbitrap-MS技术,为酸枣仁中化学成分的定性分析及质量控制提供了一种高效、快速的分析方法,为今后进一步阐明其药效物质基础及资源开发利用提供了数据基础。 This study aimed to analyze the chemical components of Zizyphi Spinosae Semen by using ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry( UHPLC-LTQ-Orbitrap-MS). The chromatographic separation of the components was achieved on a Waters Acquity UPLC BEH-C18 column( 2. 1 mm × 50 mm,1. 7 μm),with acetonitrile-water( 0. 1% formic acid) as mobile phase at a flow rate of 0. 3 m L·min^-1. Electrospray ionization-( ESI) source was applied and operated in negative ion mode. The parent ion list( PIL)-MS2 and high energy collision dissociation( HCD) techniques were used to identify triterpene saponins in Zizyphi Spinosae Semen. Through analysis of the mass spectrometry data and literature data,24 compounds and 4 unknown compounds were identified and inferred,including 21 flavonoids,5 saponins and 2 triterpenoids. The use of UHPLC-LTQ-Orbitrap-MS technology provide an efficient and rapid analytical method for the qualitative analysis and quality control of the chemical components in Zizyphi Spinosae Semen. It can also provide reference data for the research on the material basis of pharmacodynamics and the resources development and utilization.
作者 毛怡宁 武文奇 康莹 王方方 刘勇 MAO Yi-ning;WIJ Wen-qi;KANG Ying;WANG Fang-fang;LIIJ Yong(Beijing University of Chinese Medicine,Beijing 100102,China)
出处 《中国中药杂志》 CSCD 北大核心 2018年第24期4884-4891,共8页 China Journal of Chinese Materia Medica
关键词 酸枣仁 UHPLC-LTQ-0rbitrap-MS 化学成分 Zizyphi Spinosae Semen UHPLC-LTQ-Orbitrap-MS chemical components
作者简介 毛怡宁,硕士研究生,E-mail:ynmao1995@126.com;通信作者:刘勇,教授,主要从事药用植物亲缘学、中药质量与开发研究,Tel:(010)84738656,E-mail:yliu0126@aliyun.com.
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