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HPLC法测定岗梅中熊果酸和齐墩果酸的含量 被引量:1

Content Determination of Ursolic Acid and Oleanic Acid in Holly Root by HPLC
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摘要 目的:建立岗梅中熊果酸和齐墩果酸的含量测定方法。方法:采用高效液相色谱法。色谱柱为Thermo-C18,流动相为甲醇-乙腈-0.1%冰醋酸(83∶5∶12,V/V/V),流速为0.7 ml/min,柱温为30℃,检测波长为210 nm,进样量为10μl。结果:熊果酸和齐墩果酸的进样量分别在0.200 4~4.008μg(r=0.999 8)、0.100 9~2.018μg(r=0.999 6)范围内与各自峰面积呈良好的线性关系;精密度、稳定性、重复性试验的RSD〈2%;熊果酸和齐墩果酸的平均加样回收率分别为98.85%、99.44%,RSD分别为1.88%、1.83%(n均为6)。结论:该方法准确、灵敏,可为岗梅的质量控制提供参考。 OBJECTIVE:To establish a method for the content determination of ursolic acid and oleanic acid in Holly Root.METHODS:HPLC method was adopted with the column of Thermo-C18. The mobile phase was methanol-acetonitrile-0.1% glacial acetic acid(83∶5∶12,V/V/V)at the flow rate of 0.7 ml/min. The column temperature was 30 ℃,the detection wave length was set at 210 nm and the volume was 10 μl. RESULTS:There was a good linear relationship between the volume of ursolic acid and peak area in the range of 0.200 4-4.008 μg(r=0.999 8)and oleanolic acid was in the range of 0.100 9-2.018 μg(r=0.999 6). The RSDs of precision,stability,repeatability tests were less than 2%;the average recovery rates were 98.85%(RSD=1.88%,n=6)and99.44%(RSD=1.83%,n=6). CONCLUSIONS:The method is accurate and sensitive which can provide reference for quality control of Holly Root.
作者 肖彩虹 余以刚 罗苑苑 李尚 XIAO Cai-hong, YU Yi-gang, LUO Yuan-yuan, LI Shang ( 1.College of Light Industry and Food, South China University of Technology, Guangzhou 510641, China; 2.Institute for Food and Drug Inspection of Guangzhou Huangpu District, Guangzhou 510700, China; 3.Guangdong Provincial Work Injury Rehabilitation Hospital, Guangzhou 510440, China)
出处 《中国药房》 CAS 北大核心 2015年第9期1261-1263,共3页 China Pharmacy
关键词 高效液相色谱法 岗梅 熊果酸 齐墩果酸 含量测定 HPLC Holly Root Ursolic acid Oleanic acid Content determination
作者简介 副主任药师,硕士。研究方向:食品、药品成分研究及检测。电话:020-82101513。E-mail:657619206@qq.com
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