乌司他丁对百草枯诱导急性肺损伤的保护作用研究认领被引量：2

Experimental study on the Therapeutic effect of Ulinastatin against acute Lung injury by Paraquat poisoning

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摘要目的：通过建立百草枯中毒急性肺损伤大鼠模型，探讨乌司他丁（ UTI）对百草枯（ PQ）致急性肺损伤（ ALI）的保护作用及可能机制。方法将健康Wister大鼠45只随机分为三组：对照组、PQ组、UTI组。对照组：腹腔注入生理盐水；PQ组：给予含百草枯（30mg／kg）的溶液经胃管注入；UTI组：百草枯染毒后30分钟腹腔注入含乌司他丁（100000u／kg）的等容量生理盐水。每组取8小时、24小时、48小时、72小时共4个观察点，测定血液及BALF中丙二醛（ MDA）的含量、谷胱甘肽过氧化物酶（ GSH－Px）和超氧化物歧化酶（ SOD）活性。结果①PQ中毒组与对照组比较：中毒组血浆和BALF中MDA含量显著高于对照组，差异有显著性（ P＜0．01）；血浆SOD活力GSH－Px均下降，差异有显著性（ P＜0．01）；BALF中SOD、GSH－Px活力均下降，但48小时后SOD升高，差异有显著性（P＜0．01）。②UTI治疗组与PQ中毒组比较：给予UTI治疗后的治疗组与中毒组比较，血浆中MDA含量明显下降（P＜0．01），BALF中MDA含量下降（P＜0．05）；GSH－Px和SOD活力升高，差异有显著性（ P＜0．01）。结论 UTI对PQ中毒大鼠急性肺损伤具有保护作用，机制可能是减轻了肺组织的脂质过氧化反应。 Objectives To establish rats model of acute lung injury by paraquat and study the protective effect of ulinastatin （ UTI） against paraquat（ PQ）-induced acute lung injury（ ALI） and its mechanism.Methods All 45 healthy adult Wister rats were randomizedly assigned into three groups：normal control group,PQ group,UTI group.①NS group（ n=5）：the same volume of 0.9%saline solution was orally administered to rats.②PQ group（ n=20）：20% paraquat（30mg/kg） was given by the oral cavity at the first day.③UTI group（ n=20）：ulinastatin （100 000u/kg） was injected into peritoneum at 30 minutes after oral administration of PQ.The content of maleic dialdehyde （ MDA） ,glutathione peroxidase（ GSH-Px） and superoxide dismutase（ SOD） activity in blood and bronchoalveolarlavage fluid（BALF）were routinely measured at the times of 8,24,48 and 72h respectively.Results ①Com-pared with control group,the level of MDA in PQ group increased significantly both in plasma and BALF（P〈0.01）;SOD and GSH-Px in plasma were all decreased （P〈0.01）.The activities of SOD and GSH-Px in BALF were all decreased,but SOD showed in-crease 48h later（P〈0.01）.②Compared UTI group with PQ group,the MDA level decreased both in plasma and BALF（P〈0.05）, the SOD and GSH-Px activities were significantly increased as well（P〈0.01）.Conclusions Ulinastatin alleviates paraquat（PQ）-induced acute lung injury in rats,which may be attributed to its attenuation of lipid peroxidation in the tissues.

作者吴冰郑彦宏侯振才菅向东崔勇 WU Bing, ZHENG Yanhong, HOU Zhencai, JIAN Xiangdong, CUI Yong （1. Department of emergency, Shandong provincial corps hospital, Chi nese people＇s Armed PoliceForce ,JiNan 250014, China;2. Department of Emergency, Qilu hospital of Shandong university Jinan 250014, China. ）

机构地区武警山东总队医院急诊科山东大学齐鲁医院急诊科

出处《中国老年保健医学》 2015年第2期65-67,共3页

关键词乌司他丁百草枯急性肺损伤保护作用 Ulinastatin Paraquat Acute lung injury Protective effect

分类号 R595.4 [医药卫生—临床医学][医药卫生—内科学]

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