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京星黄鸡白细胞介素-2基因与H/L的关联分析 预览

The Association Analysis between IL-2 Gene and H/L in Jingxinghuang Chicken
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摘要 本研究选用鸡白细胞介素2(interleukin-2,IL-2)基因作为影响鸡免疫性状的候选基因。以京星黄鸡为研究对象,采用直接测序方法对IL-2基因的5个SNPs位点的基因型频率和等位基因频率、单倍型及其对异噬性粒细胞/淋巴细胞(H/L)指标的影响进行了研究。结果表明,所有位点在试验群体中都处于Hardy-Weinberg平衡状态,5个SNPs位点的多态性都较低,只检测到两种基因型,且纯合基因型为优势等位基因。连锁不平衡分析表明SNP1和SNP2处于完美连锁状态,SNP3、SNP4和SNP5也处于完美连锁状态。单倍型分析结果表明,京星黄鸡试验群体中共检测到4种单倍型,其中单倍型Hap1(TGACT)为优势单倍型。最小二乘分析结果表明,这5个SNPs位点不同基因型及单倍型组合对H/L的影响没有显著差异(P〉0.05)。这些结果提示以后的研究需要关注IL-2基因其他位置的SNP位点以及与更多免疫指标之间的关系,以更深入地了解IL-2在鸡免疫系统中的作用。 Interleukin-2 (IL-2) gene was regarded as a candidate gene for immune function of Jingxinghuang chicken in this study. The genotype frequencies, allele frequencies, haplotypes and association with H/L of five SNPs were studied by direct sequencing. The results indicated that all the five loci fitted Hardy-Weinberg equilibrium and showed low degrees of heterozygosity with two genotypes detected. Meanwhile, the homozygosis genotype was predominant in all the five lo- ci. Analysis of linkage disequilibrium (LD) suggested that SNP1 and SNP2 showed perfect LD, whereas SNPg,SNP4 and SNP5 showed perfect LD. Analysis of haplotypes revealed four haplo- types,among which Hap1 (TGACT) was predominant. The least square method (LSM) analysis showed that there were no significant difference between different genotypes and haplotype com- binations (P)0.05). The results suggested that more SNPs of IL-2 gene and association with ad- ditional immune traits would be investigated in future studies for better understand the roles in immune systems that chicken IL-2 playing.
作者 赵会静 张敬敬 文杰 刘冉冉 郑麦青 李庆贺 赵桂苹 ZHAO Hui-jing, ZHANG Jing-jing, WEN Jie, LIU Ran-ran, ZHENG Mai-qing, LI Qing-he, ZHAO Gui-ping (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beij ing 100193, China)
出处 《中国畜牧兽医》 CAS 北大核心 2015年第8期2098-2103,共6页 China Animal Husbandry & Veterinary Medicine
基金 基本科研业务费(2012cj-5) 中国农业科学院创新工程(ASTIP-IAS04)
关键词 IL-2基因 异噬性粒细胞/淋巴细胞 关联分析 chicken IL-2 gene heterophil/lymphocyte association analysis
作者简介 赵会静(1978-),女,河北石家庄人,硕士,研究方向:动物遗传育种,E~mail:zhhj1998927@163.com 通信作者:赵桂苹,研究员,E—mail:zhaoguiping@caas.cn
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