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组织自发荧光在大鼠局灶性脑缺血/再灌注损伤中的应用 预览 被引量:1

Application of tissue spontaneous fluorescence in draw focal cerebral ischemia reperfusion injury of rats
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摘要 目的基于特定的荧光检测技术,探讨组织自发荧光在大鼠局灶性脑缺血及再灌注损伤中的应用.方法本研究通过caliper精诺真IVIS活体成像技术检测大鼠脑组织的自发荧光.通过荧光检测,比较正常大鼠和局灶性脑缺血/再灌注损伤大鼠脑组织对应部位自发荧光强弱的变化,并进行定量分析.结果实验结果表明,局灶性脑缺血/再灌注损伤脑组织的自发荧光发生了明显变化,损伤部位的自发荧光信号明显增强,将荧光信号通过活体成像系统进行定量,与正常脑组织比较差异有统计学意义,P〈0.01.结论大鼠脑组织在局灶性脑缺血/再灌注损伤后,脑组织的自发荧光明显增强,为大鼠局灶性脑缺血/再灌注损伤模型的研究及其评价提供新的方法. Aim To discuss the application of tissue spontane-ous fluorescence for draw focal cerebral ischemia reperfusion in-jury in rats based on specific fluorescence detection technology. Methods The change of spontaneous fluorescence WAS COM-PARED between the brains of normal rats and those of rats with draw focal cerebral ischemia-reperfusion injury and an quantita-tive analysis was then made. Result The results showed that spontaneous fluorescence of brain tissue for focal cerebral ische-mia reperfusion injury changed significantly. Spontaneous fluo-rescence signal of injury considerably enhanced. The fluores-cence signal which was quantified by IVIS had significant statisti-cal significance compared with normal brain tissue, P <0. 05. Conclusion Our research shows that spontaneous fluorescence of brain tissue enhances obviously after focal cerebral ischemia-reperfusion injury. Our research provides a method for the re-search and evaluation of focal cerebral ischemia-reperfusion inju-ry model in rats.
作者 田树红 王日超 邢桂兰 符健 TIAN Shu-hong;WANG Ri-chao;XING Gui-lan;FU Jian;Hainan Research Center for Drug Safety Evaluation,Hainan Medical University
出处 《中国药理学通报》 CAS CSCD 北大核心 2015年第10期1473-1476,共4页 Chinese Pharmacological Bulletin
基金 国家自然科学基金资助项目(No81160408)
关键词 大鼠 脑组织 自发荧光 局灶性脑缺血/再灌注 活体成像系统 rat brain tissue autofluorescence focal cerebral ischemia reperfusion in vivo imaging system
作者简介 田树红(1980-),男,硕士,助理研究员,研究方向:药理学与毒理学、抗肿瘤药效学,E-mail:18089862900@163.com 符健(1960-),男,博士,教授,研究方向:药理毒理学,通讯作者,Tel:0898-66982118,E-mail:Fujian.hnmc@163.com
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