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表松脂醇抑制巨噬细胞泡沫化的作用及潜在机制研究 被引量:1

Inhibition of epipinoresinol on macrophage foam and potential mechanisms
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摘要 目的探究表松脂醇在抗巨噬细胞泡沫化方面的作用及潜在机制。方法采用倒置显微镜拍照和用酶标仪检测吸光度等方法,评价表松脂醇对泡沫细胞形成的抑制作用。采用荧光检测法检测胆固醇流入及流出情况,用实时荧光定量PCR(RT-PCR)检测流出、流入相关基因的表达情况。结果表松脂醇能够显著抑制RAW264.7巨噬细胞由氧化低密度脂蛋白(ox-LDL)诱导的胆固醇累积,能够显著增强由高密度脂蛋白(HDL)介导的胆固醇流出,同时还能够显著抑制胆固醇的流入。RT-PCR结果表明,表松脂醇能够上调过氧化物增殖激活受体γ(PPARγ)、肝X受体α(LXRα)、ATP结合盒转运子A1(ABCA1)和ATP结合盒转运子G1(ABCG1)基因m RNA的水平,下调清道夫受体A1(SR-A1)和A2(SR-A2)基因m RNA的水平。结论表松脂醇是一种新的泡沫细胞形成抑制剂,其作用可能是通过上调PPARγ-LXRα-ABCA1/ABCG1通路和下调SR-A1、SR-A2实现的,其可能在防治动脉粥样硬化方面具有潜在的作用。 Objective To clarify the potential inhibitive effect of epipinoresinol on macrophage foam and potential mechanisms. Methods The inhibition of epipinoresinol on foam cell formation after stained with oil red O was assessed by Image-Pro Plus and Microplate Reader, and the effect on cholesterol efflux and influx was tested by fluorescence detection to obtain cholesterol inflows-time curve and outflow rate. Additionally the cholesterol flow-associated genes expression was checked by real-time PCR(RT-PCR). Results Epipinoresinol dose-dependently inhibited the enhanced cholesterol accumulation elicited by oxidized low-density lipoprotein cholesterol(ox-LDL) in RAW264.7 cells. Treatment with epipinoresinol significantly enhanced the cholesterol efflux mediated by high-density lipoprotein(HDL) and substantially inhibited the cholesterol influx. RT-PCR showed that epipinoresinol significantly increased the m RNA levels of PPARγ, LXRα, ABCA1, and ABCG1, decreased those of SR-A1 and SR-A2. Conclusion Epipinoresinol is a new inhibitor on foam cell formation that may stimulate the cholesterol efflux through up-regulating the PPARγ-LXRα-ABCA1/ABCG1 pathway and prevent cholesterol influx through down-regulating SR-A1 and SR-A2, which may be useful on atherosclerosis treatment.
作者 王帅 刘明玥 张雪 李欣 郭鹏 吴崇明 WANG Shuai,LIU Ming-yue,ZHANG Xue,LI Xin,GUO Peng,WU Chong-ming(1. Pharmacology and Toxicology Research Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China; 2. Research Centre on Life Sciences and Environment Sciences, Harbin University of Commerce, Harbin 150076, China)
出处 《中草药》 CAS CSCD 北大核心 2015年第18期2737-2742,共6页 Chinese Traditional and Herbal Drugs
基金 国家自然科学基金资助项目(81001437) 北京市自然科学基金资助项目(7102111)
关键词 表松脂醇 动脉粥样硬化 泡沫细胞 胆固醇流入 胆固醇流出 巨噬细胞 epipinoresinol atherosclerosis foam cell cholesterol influx cholesterol efflux macrophage
作者简介 王帅(1987-),男,在读博士研究生,研究方向为中药药理学。Tel:18600466676 E-mail:zhuizhirun@163.com 通信作者 郭鹏,男,博士,副研究员,硕士生导师,研究方向为心脑血管药理学。Tel:(010)57833235 E-mail:pguo@implad.ac.cn 通信作者 吴崇明,男,博士,副研究员,研究方向为天然药物药理学。Tel:(010)57833235 E-mail:cmwu@implad.ao.cn
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  • 1Yu X H, Fu Y C, Zhang D W, et al. Foam cells in atherosclerosis [J]. CIin China Acta, 2013,424(9): 245-252. 被引量:1
  • 2Glass C K, Witztum J L. Atherosclerosis: the road ahead [J]. Cell, 2001, 104(4): 503-516. 被引量:1
  • 3Darwish H A, Arab H H, Abdelsalam R M. Epipinoresinol alleviates testicular dysfunction in adjuvant arthritic rats via suppression of inflammation and apoptosis: Comparison with celecoxib [J]. Toxicol Appl Pharmacol, 2014, 279(2): 129-140. 被引量:1
  • 4Tabas I. Apoptosis and plaque destabilization in atherosclerosis: the role of macrophage apoptosis induced by cholesterol [J]. Cell Death Differ, 2004, 11(7): 12-16. 被引量:1
  • 5Ross R. Atherosxlerosis-an inflammatory disease [J]. N EngIJMed, 1999, 340(2): 115-126. 被引量:1
  • 6Pennings M, Meurs I, Ye D, et al. Regulation of cholesterol homeostasis in macrophages and consequences for atherosclerotic lesion development [J]. FEBS Lett, 2006, 580(23): 5588-5596. 被引量:1
  • 7Reiss A B, Cronstein B N. Regulation of foam cells by adenosine [J]. Arterioscler Thromb Vasc Biol, 2012, 32(4): 879-886. 被引量:1
  • 8Zhang M, Wu J F, Chen W J, et aI. MicroRNA-27aJb regulates cellular cholesterol effiux, influx and esterificationJhydrolysis in THP-1 macrophages [J]. Atherosclerosis, 2014, 234(1): 54-64. 被引量:1
  • 9Ohashi R, Mu H, Wang X, et al. Reverse cholesterol transport and cholesterol effiux in atherosclerosis [J]. QJM, 2005, 98(12): 845-856. 被引量:1
  • 10Dean M, Hamon Y, Chimin G. The human ATP-binding cassette (ABC) transporter superfamily [J]. J Lipid Res, 2001, 42(7): 1007-1017. 被引量:1

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