目的：探讨碳化二亚胺盐酸盐[1-ethyl-3-（3-dimethylaminopropyl）carbodiimide,EDC]乙醇溶液表面处理对牙本质即刻微拉伸粘接强度、断裂模式和粘接界面微观形态的影响。方法：称取不同质量EDC溶解于无水乙醇中,配制不同浓度（2、1、0.3、0.1、0.01 mol/L）的EDC乙醇溶液。将28颗人离体第三磨牙随机平分为7组：5种浓度的EDC处理组、未处理组、乙醇溶剂处理组。在低速精密切割机下去除牙合面牙釉质,暴露中层牙本质,35%（质量分数）磷酸凝胶酸蚀后根据不同分组对脱矿牙本质表面分别进行处理60 s,使用全酸蚀粘接剂（Single Bond 2）粘接处理后,堆塑复合树脂并固化。室温下保存24 h后,制备标准条形试件并测定微拉伸粘接强度,在体视显微镜下对试件的断裂模式进行观察。结果：2 mol/L组[（22.17±13.31）MPa]和1 mol/L组[（45.31±17.80）MPa]的粘接强度显著下降（P〈0.05）,粘接断裂模式发生的比例明显提高,分别为81.2%和41.3%。其余各组粘接强度差异无统计学意义（P〉0.05）。结论：0.3、0.1和0.01 mol/L的EDC乙醇溶液表面处理对牙本质即刻粘接强度无明显影响,2 mol/L和1 mol/L的EDC乙醇溶液表面处理会显著降低牙本质即刻粘接强度。
Objective： To evaluate the microtensile bond strength changes and patterns of fractures of the bonding interface after dentine surface treatment with carbodiimide-ethanol solution. Methods： 1-ethyl-3-（ 3-dimethylaminopropyl） carbodiimide（ EDC） dissolved in ethanol was diluted into different concentrations of 2,1,0. 3,0. 1 and 0. 01 mol / L EDC-ethanol solutions. Twenty-eight caries-free extracted human third molars were ground metallurgically to prepare flat occlusal mid-coronal dentin surfaces and etched with 35%（ mass fraction） phosphoric acid gel. Then they were treated with EDC-ethanol solution for 60 s before the bonding procedure and randomly divided into five experimental groups corresponding to the tested EDC-ethanol concentrations. The ethanol treated and no pre-treated surfaces were used as controls. Single Bond 2 adhesive was applied and resin composite disk was stacked on the treated dentine surface. The teeth with resin composite disks were stored in water at room temperature for 24 h and then sectioned longitudinally to produce stick specimens for microtensile bond strength test. Fracture patterns were observed with a stereomicroscope. Results： The dentin surfaces pre-treated with 2 mol / L [（ 22. 17 ±13. 31） MPa] and 1 mol / L [（ 45. 31 ± 17. 80） MPa]EDC-ethanol solutions resulted in statistically significant lower bond strength value（ P〈0. 05）. Increasing numbers of fracture pattern at the resin-dentin interface were also found in this two groups with percentages of 81. 2% and 41. 3% respectively. No significant difference was observed in the groups with 0. 3,0. 1,0. 01 mol / L EDC surface treatment（ P〈0. 05）. Conclusion： No significant difference of immediate bond strengths was found in the 0. 3,0. 1,0. 01 mol / L groups compared with the control group. EDC-ethanol solution surface treatment with concentrations of 2 mol / L and 1 mol / L resulted in decreasing of the bonding strength.
Journal of Peking University：Health Sciences