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健脾补肾方对BALBc鼠大肠癌肝转移VEGF表达影响的实验研究 预览 被引量:1

Exper imental Study of Jianpi Bushen Decoction on the Expression of VEGF in BALBc Rat with Liver Metastasis of Colorectal Cancer
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摘要 目的探讨健脾补肾方对BALBc鼠大肠癌肝转移血管内皮生长因子(VEGF)表达影响性。方法先对BALBc鼠大肠癌肝转移进行模型,随机分成环磷酰胺组、生理盐水组和健脾补肾方组,各组均进行各自药物灌胃处理,每日1次,共2周,检测每组VEGF表达情况。结果抑瘤率上,生理盐水组为0,环磷酰胺组为35.40%,健脾补肾方组为29.90%,3组两两比较差异有统计学意义(P〈0.05);脾脏指数上,生理盐水组为(8.97±0.31)mg/g,环磷酰胺组为(8.22±0.14)mg/g,健脾补肾方组为(9.11±0.13)mg/g,生理盐水组和环磷酰胺组、环磷酰胺组和健脾补肾方组比较差异有统计学意义(P〈0.05);VEGF表达率上,生理盐水组"-""+""++"分别占8.33%、8.33%、83.33%,环磷酰胺组分别占41.67%、25.00%、33.33%,健脾补肾方组分别占33.33%、25.00%、41.67%,环磷酰胺组和健脾补肾方组VEGF表达比较差异无统计学意义(P〉0.05),而生理盐水组和环磷酰胺组、生理盐水组和健脾补肾组比较差异有统计学意义(P〈0.05)。结论健脾补肾方能通过降低BALBc鼠大肠癌肝转移VEGF表达提高临床效果。 Objective: To investigate the effects of Jianpi Bushen Decoction on the expression of VEGF in BALBc rat with liver metastasis of colorectal cancer. Methods: At first,BALBc mouse model was made with liver metastasis of colorectal cancer,and randomly divided into the cyclophosphamide group,the saline group and Jianpi Bushen Decoction groups. Each group was treated with drug perfusion once per day,lasting for 2 weeks,and the expression of VEGF was detected. Results: As for the inhibition rate,the saline group was 0,the cyclophosphamide group 35.4%,Jianpi Bushen Decoction group 29.9%,with statistical significance(P〈0.05);as for the spleen index,the saline group was(8.97 ±0.31) mg/g,the cyclophosphamide group(8.22 ±0.14) mg/ g,Jianpi Bushen Decoction group(9.11±0.13) mg/g;there was statistical significance between the saline group and the cyclophosphamide group,and between the cyclophosphamide group and Jianpi Bushen Decoction group(P〈0.05).As for the expression of VEGF,-,+,+ of the saline group was respectively 8.33%,8.33% and 83.33%,the cyclophosphamide group 41.67%%,25.00% and 33.33%,Jianpi Bushen Decoction group 33.33%,25%,41.67%.The difference in the cyclophosphamide group and Jianpi Bushen Decoction group in VEGF expression was not statistically significant(P〉0.05),while the difference between the two groups was statistically significant(P〈0.05). Conclusion: spleen and kidney in order by reducing BALBc mouse VEGF expression in colorectal liver metastases improve clinical results.
作者 金妙妙 傅健飞 JIN Miaomiao,FU Jianfei(Central Hospital of Jinhua City,Zhejiang Provinc e, Zhejiang , Jinhua 321000, China)
出处 《中国中医急症》 2015年第11期1906-1907,1973共3页 Journal of Emergency in Traditional Chinese Medicine
基金 浙江省自然科学基金项目(LY13H190006)
关键词 健脾补肾方 BALBc鼠 大肠癌肝转移 血管内皮生长因子 Jianpi Bushen Decoction BALBc mouse Liver metastasis of colorectal cancer Vascular endothelial growth factor
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