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高效液相色谱法测定人血浆中游离安妥沙星浓度研究 被引量:4

Determination of unbound antofloxacin in human plasma by high performance liquid chromatography
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摘要 目的建立高效液相色谱法测定人血浆中游离安妥沙星浓度的方法。方法用超离心法进行样品处理,色谱柱为Alltima C18柱(150 mm×4.6mm,5μm),柱温为室温,流动相为乙腈-0.05 mol·L-(-1)磷酸盐缓冲液(p H3.0)=13∶87,流速为1.0 m L·min-(-1),波长为293 nm,进行专属性、标准曲线与定量下限、精密度与回收率、稳定性考察。结果游离安妥沙星在0.08-5.49μg·m L-(-1)呈现良好线性关系,日内和日间精密度相对标准偏差(RSD)2.78%-10.03%,回收率为92.09%-108.96%。结论本方法快速,精密,准确测定游离安妥沙星,可用于受试者给药后安妥沙星的测定。 Objective To develop an analytical method for analyzing the unbound concentration of antofloxacin in human plasma. Methods A HPLC method was developed to determine the unbound concentration of antofloxacin in human plasma using ultra-centrifugation. The column was Alltima C18( 150 mm × 4. 6 mm,5 μm) with room temperature as the column temperature. The mobile phase was composed of 13 ∶ 87 acetonitrile-0. 05 mol · L~(-1)phosphate buffer( p H 3. 0) with detection wavelength set at 293 nm,and the flow rate was 1. 0 m L · min~(-1). The method was validated for specificity,precision and accuracy,recovery as well as stability. Results The calibration curves were linear over the range of 0. 08-5. 49 μg·m L~(-1). The intra-day and inter-day precision evaluated at lower limit of quantification and quality control levels were within 2. 78%~(-1)0. 03%. The recoveries calculated for the antofloxacin were within 92. 09%~(-1)08. 96% from spiked plasma ultrafiltrate samples. Conclusion The analytical method is quick, precise and accuracy and could be used in the analysis of unbound concentration of antofloxacin in plasma.
作者 魏敏吉 吕媛 赵彩芸 石利维 康子胜 WEI Min-ji,L Yuan,ZHAO Cai-yun,SHI Li-wei,KANG Zi-sheng(Institute of Clinical Pharmacology, First Hospital of Peking University, Beijing 100034, China)
出处 《中国临床药理学杂志》 CAS CSCD 北大核心 2016年第1期65-67,共3页 The Chinese Journal of Clinical Pharmacology
基金 科技部脑血管疾病和耐药性病原菌感染的国际化新药临床评价研究技术平台基金资助项目(2008ZX09312-017)
关键词 安妥沙星 游离药物浓度 药代动力学 血浆 antofloxacin unbound concentration pharmacokinetic plasma
作者简介 魏敏吉(1964-),男,博士,硕士生导师,主要从事药代动力学和临床药理学研究 通信作者:吕媛,研究员,博士生导师Tel:(010)82802315E-mail:lyzx5857@163.com
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  • 1叶辉,武济民,杨玉社,陈凯先,嵇汝运.左旋氧氟沙星衍生物(YH54,YH57)体外抗菌活性[J].中国药理学通报,2002,18(1):112-113. 被引量:6
  • 2Xiao YH, Lv Y, Kang ZS, et al. Tolerability and pharmaeokinetics of antofloxacin hydrochloride after multiple oral dose administration in healthy Chinese male volunteers [ J ]. lnt J Clin Pharmacol Ther, 2008,46 : 172 - 179. 被引量:1
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