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STIM1基因沉默对人下咽癌细胞系FaDu裸鼠成瘤的影响 被引量:2

STIM1 gene silencing suppresses tumor formation of human hypopharyngeal carcinoma cell line FaDu in nude mice
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摘要 目的研究STIM1基因沉默对人下咽癌细胞系FaDu裸鼠成瘤的影响。方法利用siRNA慢病毒感染的方法沉默人下咽癌细胞系FaDu中STIM1基因的表达,采用Real—timePCR和Westernblot分别从mRNA和蛋白质水平检测抑制效果。将10只裸鼠计算机随机分成两组,每组5只,于皮下接种下咽癌细胞。抑制组:接种STIM1-siRNA慢病毒感染的下咽癌细胞;对照组:接种阴性对照慢病毒感染的下咽癌细胞。分别在种瘤后的第10天、14天、18天、22天测量裸鼠瘤体体积,并利用NightOWL分子成像系统进行小动物成像实验,于种瘤后第22天人道方法处死裸小鼠并测量瘤体重量。本实验采用独立样本t检验,P〈0.05为差异有统计学意义。结果STIM1-siRNA慢病毒感染后的FaDu细胞中STIMI的表达受到明显抑制。4个时间点对实验组裸鼠的瘤体体积的测量结果分别为(40±35)、(106±41)、(340±158)和(917±252)mm3;对照组分别为(51±25)、(262±107)、(716±226)和(1682±592)mm3,抑制组明显小于对照组(P〈0.05)。对照组和抑制组的瘤体重量分别为(1.22±0.d1)g和(0.66±0.26)g,对照组明显重于实验组(P〈0.05)。小动物成像的结果显示抑制组裸鼠瘤体的荧光范围及信号强度值均小于对照组。结论运用慢病毒感染方法能够有效抑制人下咽癌细胞系FaDu中STIM1的表达,沉默STIM1后的FaDu在体内的生长和活性也受到明显抑制。 Objective To evaluate the inhibition effect of STIM1 gene silencing on tumor growth of human hypopharyngeal carcinoma cell lines FaDu in nude mice. Methods STIM1 gene in FaDu was silenced by lentiviral infection, and the effect of inhibition was detected by Real-time PCR and Western blot after lentiviral infection. Nude mice were divided into 2 groups, 5 mice in each group. Inhibition group: subcutaneous inject FaDu cells which STIM1 expression was inhibited. Control group: subcutaneous inject FaDu cells infected with negative control siRNA-expressing lentivirus. Tumor volumes were measured by calipers,and small animal imaging was detected by NightOWL system on the day 10, 14, 18 and 22 after tumor inoculated. Tumor weights were evaluated in the day 22 after tumor inoculated. Statistical analysis was performed using standard student test (P value threshold was 0. 05 ). Results The expressions of human STIM1 gene and protein in FaDu cells were suppressed effectively after STIMI-siRNA lentiviral infection. The mean tumor volumes of control group and inhibition group were (51 ± 25)mm3 and (40±35) mm3, respectively, on the day 10, (262± 107) and (106±41) mm3 on the day 14, (716 ±226) and (340±158 ) mm3 on the day, ( 1 682 ± 592) mm3 and (917 ± 252) mm3 on the day 22 (P 〈 0.05). On the day 22, the tumor weight was ( 1.22 ±0. 41 ) g in control group and ( 0. 66 ±0. 26 ) g in STIMI-siRNA group ( P 〈 0. 05). Small animal imaging showed that the tumors had a smaller fluorescence range with lower signal intensity in STIMI-siRNA group than in control group on the day 14, 18 and 22. Conclusion The expression of STIM1 in human hypopharyngeal carcinoma cell lines FaDu can be inhibited effectively by lentiviral infection, causing the inhibition of tumor formation and growth.
作者 崔晓波 孙源昊 武帅 王军 白云飞 王亚平 王博谦 Cui Xiaobo, Sun Yuanhao, Wu Shuai, Wang Jun, Bai Yunfei, Wang Yaping,Wang Boqian(Department of Otorhinolargngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot 010050, China)
出处 《中华耳鼻咽喉头颈外科杂志》 CAS CSCD 北大核心 2016年第1期68-72,共5页 Chinese JOurnal of Otorhinolaryngology Head and Neck Surgery
基金 内蒙古自然科学基金(2014MS0856)
关键词 下咽肿瘤 膜蛋白质类 肿瘤蛋白质类 基因沉默 小鼠 Hypopharyngeal neoplasms Membrane proteins Neoplasm proteins Genesilemcing Mice,mude
作者简介 通信作者:崔晓波,Email:nmcxb@sina.com.cn
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