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壳聚糖温敏性水凝胶蛋白载体的制备及其性能研究 预览 被引量:2

Preparation and Properties of Chitosan Thermosensitive Hydrogels for Protein Drug Delivery
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摘要 为了增强蛋白类药物的活性和寿命,应用物理交联技术制备了壳聚糖(CS)/京尼平(GP)/明胶(G)/β-甘油磷酸钠(β-GP)共混的温敏性水凝胶,采用红外光谱(FT-IR)、透射电镜(SEM)及降解实验对壳聚糖水凝胶的理化性能进行了表征;并研究了其对牛血清蛋白(BSA)的释放规律.结果显示:壳聚糖温敏性水凝胶的凝胶时间较短,约为50-126s;透射电镜显示4种物质相互交联为互通的多孔网络,结构致密均一;壳聚糖水凝胶具有一定的降解能力;BSA释放时间长达170h,最终释放率为76%-88%,蛋白释放曲线符合药物释放模型. In order to enhance the activity and life-span of protein,which was applied a thermosensitive chitosan(CS)/genipin(GP)/genlatin(G)/β-glycerophosphate(β-GP)hydrogel was developed using physical crosslinking method.The physicochemical properties of chitosan hydrogel was confirmed by infrared spectroscopy(FT-IR),transmission electron microscopy(SEM)and degradation test,its ability to release BSA.The results showed that the gel time of chitosan thermosensitive hydrogel is short,about 40—140s;The SEM revealed that the four substances crosslinked to porous network with interworking and dense homogeneous;Chitosan hydrogel has a certain ability to degrade;The BSA release time up to 170 hand the final release rate was 76%—88%,Protein release profiles are consistent with the model.
作者 楚立凯 岳凌 杨占山 OHU Likai, YUE Ling, YANG Zhanshan (School of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China)
出处 《复旦学报:自然科学版》 CAS CSCD 北大核心 2015年第5期609-615,共7页 Journal of Fudan University(Natural Science)
基金 国家自然科学基金(81372922)
关键词 温敏性水凝胶 壳聚糖 京尼平 药物缓释 牛血清蛋白 temperature-sensitive hydrogel chitosan(CS) genipin(GP) drug delivery bovine serum albumin(BSA)
作者简介 楚立凯(1988-),男,硕士研究生; 杨占山,教授,通讯联系人,E-mail:fd@suda.edu.cn.
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