Objectives： To observe the effect of isoflurane general anesthetic on sperm movement function,in vitro capacitation and sperm acrosome reaction,so as to provide basis for clinical application. Methods： The sifted 18 specimens of normal sperm from male sperm samples in male hospital outpatient clinic were selected and each sperm sample was divided into three parts. In three test tubes,respectively,they were given 1. 1% isoflurane（ ISO1）,2. 2% isoflurane（ ISO2） and air as the control group. All the organic glass boxes were placed in incubator for 5 h cultivation to evaluate sperm movement and acrosome reaction. Results： After 5 h of 1. 1% and 2. 2%isoflurane treated on sperm,compared with control group,the sperm vitality,linear velocity,the curve speed and average speed significantly decreased,with statistically significant difference（ P〈0. 05）. Compared with ISO1 and ISO2 group,ISO2 group decreased more obviously,which presented a dose- response relationship. Namely with the increase of concentration,the inhibition effect on sperm motion function was more obvious. For the influence of capacitation,compared with control group,both kinds of concentrations of isoflurane could obviously inhibit in vitro capacitation,with statistically significant difference（ P〈0. 05）. ISO1 and ISO2 group had no significant difference,P〉0. 05. The percentages of the sperm acrosome reaction of the two groups were also significantly increased before treatment,with statistically significant difference（ P〈0. 05）. But there was no significant difference between the two groups after treatment. Conclusion： Isoflurane can inhibit sperm movement and capacity. With the increase of concentration,the inhibition increases and Isoflurane has no obvious effect on sperm body reaction.
The Chinese Journal of Human Sexuality