目的对2010年-2014年江苏省急性驰缓性麻痹（AFP）病例粪便标本中非脊髓灰质炎其他肠道病毒（NPEV）监测结果进行分析,为疾病的预防控制和早期诊断治疗提供实验室数据。方法按照世界卫生组织（WHO）《脊髓灰质炎病毒检验手册》操作规程进行病毒分离与鉴定。SPSS 18.0统计软件进行统计分析。结果共检测1 369例AFP以及162份AFP接触者的粪便标本,共分离出72株NPEV。各年度分离率差异无统计学意义,且AFP病例相关非脊髓灰质炎肠道病毒的分离率与年龄分布无关,不同月份组间AFP病例相关非脊髓灰质炎肠道病毒分离率差异有统计学意义（P〈0.05）,NPEV感染相关AFP病例7月-9月最高。结论开展AFP病例相关非脊髓灰质炎肠道病毒监测,尤其是病原体的分型鉴定,对于维持无脊髓灰质炎的目标及完善全省急性迟缓性麻痹病例的应急处置能力有着积极意义。
Objective To analyze the results of non- polio enterovirus（ NPEV） among acute flaccid paralysis（ AFP） cases in Jiangsu during 2010- 2014,so as to provide laboratory data for prevention and control as well as the early diagnosis and treatment. Methods The viruses were isolated and identified according to Polio Laboratory Manual. SPSS 18. 0 software was used for statistical analysis. Results Feces specimens were collected and detected from 1 369 AFP patients and 162 AFP contact during 2010- 2014,and total 72 strains of NPEV were isolated. There were no statistical significance on the differences of separation rates of NPEV among different ages. The separation rate of NPEV in AFP cases had nothing to do with age distribution,and the difference on the isolation rates of NPEV related to AFP cases in different months was statistically significant（ P〈0. 05）. Statistical analysis showed that the NPEV associated AFP cases occurred mainly during July- September. Conclusion To carry out the monitoring of NPEV associated with AFP cases,especially the identification of the pathogen,has positive significance for maintaining the target polio- free status,and improving the emergency response ability of the province＇s acute delayed paralysis case.
Chinses Journal of Health Laboratory Technology