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石菖蒲-远志药对不同组分对AD模型大鼠学习记忆和海马突触蛋白表达的影响 被引量:13

Effect of Different Components of Acori Tatarinowii Rhizoma-Polygalae Radix on Learning and Memory Capacity and Hippocampal Synaptic Proteins Expression in AD Model Rats
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摘要 目的:探讨石菖蒲-远志药对不同组分(挥发油、水提物)对阿尔兹海默病(AD)模型大鼠学习记忆和海马突触蛋白表达的影响。方法:选用SPF级SD大鼠,随机分为8组,分别为正常组、痴呆模型组、石菖蒲-远志挥发油低、中、高剂量组和石菖蒲-远志水提物低、中、高剂量组。用D-半乳糖(D-gal)制成亚急性衰老模型,造模成功后治疗组大鼠分别给予石菖蒲-远志不同组分0.6,1.2,1.8 g·kg-1(生药用量)体重ig,正常组和模型组分别给相应生理盐水ig,给药28 d后,用Morris水迷宫实验测试大鼠的空间学习记忆能力,蛋白质免疫印迹(Western blot)检测各组大鼠海马突触蛋白含量。结果:与正常组比较,模型组潜伏期延长(P〈0.01),药物治疗组与模型组比较,潜伏期缩短(P〈0.01),石菖蒲-远志药对水提物高剂量组潜伏期短于其低剂量组和挥发油中、低剂量组(P〈0.05);空间探索实验中水提物高剂量组目标象限时间明显优于其低剂量组(P〈0.01),且优于挥发油各治疗组(P〈0.05);石菖蒲-远志药对两种组分均可使AD模型大鼠海马内突触蛋白突触素(Synaptophysin),突触蛋白1(Synapsin1),突触后致密物-95(PSD-95),PSD-93蛋白表达高于D-gal模型组(P〈0.05),并呈一定的剂量依赖性,但挥发油组分的Synapsin1表达却随着剂量的增加而递减。结论:石菖蒲-远志药对能促进AD模型大鼠海马内突触蛋白的表达,尤以水提物组分效果明显。 Objective: To investigate the effect of different components of Acori Tatarinowii RhizomaPolygalae Radix( volatile oil,aqueous extract) on learning and memory capacity as well as hippocampal synaptic proteins expression in Alzheimer's disease( AD) model rats. Method: Sprague Dawley rats( Grade: SPF) were randomly divided into 8 groups: normal group, dementia model group, Acori Tatarinowii Rhizoma-Polygalae Radix volatile oil low, middle, high dosage groups. and Acori Tatarinowii Rhizoma-Polygalae Radix aqueous extract low,middle,high dosage groups. Subacute aging models were established by D-galactose( D-gal). After successful modeling, the rats in treatment groups were given with different components of Acori Tatarinowii Rhizoma-Polygalae Radix 0. 6,1. 2,1. 8 g·kg- 1( crude drug dosage) by gastric lavage; while the rats in normal group and model group were given with corresponding dose of normal saline. The 28 days later,their ability of spatial learning and memory was tested by Morris water maze in rats,and hippocampal synaptic protein levels in rats of various groups were detected by Western blot. Result: Compared with normal group,the latency period was prolonged in model group( P〈 0. 01). Compared with the model group,the latency period was shortened in drug treatment groups( P〈 0. 01). The latency period in Acori Tatarinowii Rhizoma-Polygalae Radix aqueous extract high dose group was shorter than that in low dose group and all volatile oil groups( P〈 0. 05). In spatial probe test,aqueous extract high dose group was significantly superior to its low dose group( P〈 0. 01) and all volatile oil groups( P〈 0. 05). Both components of Acori Tatarinowii Rhizoma-Polygalae Radix can make the expressions of Synaptophysin, Synapsin1, PSD-95, and PSD-93 in AD model rats higher than those of D-gal model rats( P〈 0. 05), with a dose-dependent relationship, but the Synapsin1 expression of volatile oil was decreased with the increase of dose. Conclusion:
作者 张智华 王秀莲 吴建红 吕银娟 柳琳 ZHANG Zhi-hua,WANG Xiu-lian,WU Jian-hong,LYU Yin-juan,LIU Lin(College of Basic Medical,Hubei University of Traditional Chinese Medicine, Wuhan 430065, China)
出处 《中国实验方剂学杂志》 CAS CSCD 北大核心 2016年第7期120-124,共5页 China Journal of Experimental Traditional Medical Formulae
基金 国家自然科学基金项目(81202734)
关键词 石菖蒲 远志 挥发油 水提物 学习记忆 海马 突触蛋白 Acori Tatarinowii Radix Polygalae Radix volatile oil aqueous extract learning and memory capacity hippocampus synaptic proteins
作者简介 张智华,博士,副教授,硕士生导师,主要从事方剂配伍规律及临床应用研究,Tel:027-68890141,E-mail:zzhor@163.com
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