期刊文献+

外源H_2O_2对低温胁迫下柑橘叶片抗寒性的影响 预览 被引量:10

Effect of Exogenous H_2O_2 Treatment on Cold Resistance of Citrus reticulata Leaves under Low Temperature Stress
在线阅读 下载PDF
收藏 分享 导出
摘要 以柑橘品种‘日南1号’离体秋稍为试材,采用Hoagland营养液培养方法,研究添加不同浓度H_2O_2处理对4℃低温胁迫下柑橘生长状态和叶片细胞相对电导率(REC)、丙二醛(MDA)含量、脯氨酸含量以及过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性等生理指标的影响,筛选缓解柑橘低温伤害的最佳H_2O_2处理浓度,探讨外源H_2O_2处理对柑橘耐寒能力影响机制。结果显示:随低温胁迫时间的延长,各处理组柑橘叶片卷曲和叶片细胞膜伤害程度均逐渐加重;外源施加0.2和1.0mmol·L-1 H_2O_2处理均能缓解低温胁迫引起的叶片卷曲和萎蔫,降低叶片中REC和MDA的升高,减少叶片细胞中内源H_2O_2的积累,提高渗透调节物质脯氨酸的含量和抗氧化酶SOD、CAT和POD的活性,并以1.0mmol·L-1 H_2O_2缓解效果更为显著。研究表明,4℃低温能够引起柑橘离体秋梢叶片卷曲、枯萎、脱落和细胞膜伤害症状,外源1.0mmol·L-1 H_2O_2可以通过提高叶片的脯氨酸含量和SOD、CAT和POD抗氧化酶活性,有效缓解低温对柑橘叶片细胞膜的伤害,从而增强其抗寒性。 Fall shoot of Citrus(Citrus reticulata Blanco.‘Nichinan No.1')cultured in hoagland hydroponic solution were used as experimental materials to study effects of different concentration of H_2O_2 treatments on growing state and physiological indexes of relative electric conductivity(REC),contents of malondialdehyde(MDA)and proline as well as activities of catalase(CAT),superoxide dismutase(SOD)and peroxidase(POD)in C.reticulataleaves under 4 ℃low temperature stress.Meantime optimum H_2O_2 concentration was selected to alleviate chilling injury of C.reticulataand the mechanism of tolerance to cold in C.reticulata with exogenous H_2O_2 treated was also explored.Results showed that the degree of curl and cell membrane damage of leaf gradually increased with the extension of low temperature stress time in all treated groups of C.reticulata.Both 0.2mmol·L-1and 1.0mmol·L-1concentrations of H_2O_2 applied to hydroponic solution could ease C.reticulataleaves curling and wilting caused by low temperature stress,lower the increase of REC and MDA,reduce the accumulation of H_2O_2,and increase the content of osmotic proline and activities of SOD,CAT and POD in C.reticulataleaves.The 1.0mmol·L-1 of H_2O_2 alleviated chilling stress on C.reticulatadamage more significant than that of the concentration of 0.2mmol·L-1.The studies confirmed that the symptoms of leaf curl,wither,fall off and cell membrane damage were caused by treatment of 4℃low temperature in autumn shoots leaves of C.reticulata.And the treatment of1.0mmol·L-1H_2O_2 could increase the content of proline and activities of SOD,CAT and POD under low temperature stress,and effectively relieve the damage of cell membrane under low temperature in C.reticu-lataleaves,to enhance its cold resistance.
作者 蒋景龙 JIANG Jinglong (School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723001,China)
出处 《西北植物学报》 CAS CSCD 北大核心 2016年第3期499-505,共7页 Acta Botanica Boreali-Occidentalia Sinica
基金 陕西省科技厅自然科学基础研究计划(2014JQ3113) 陕西省教育厅自然科学研究项目(14JK1158) 陕西理工学院科研基金(SLGKY15-41) 陕西理工学院研究生创新基金(SLGYCX1517)
关键词 柑橘 低温胁迫 外源过氧化氢 抗寒性 Citrus reticulata low temperature stress exogenous hydrogen peroxide cold resistance
作者简介 蒋景龙(1980-),男,博士,讲师,硕士生导师,主要从事逆境植物学方面的研究。E—mail:jiangjinglong511@163.com
  • 相关文献

参考文献20

  • 1李新生,江海,郭念文编著..陕西柑橘资源与产业化开发研究[M].北京:科学出版社,2014:357.
  • 2陈志远,邸丽俊,王国栋,黄新民,李新生,蒋景龙.柑橘冻害抵抗分子机理研究进展[J].北方园艺,2014(1):188-191. 被引量:5
  • 3JIANG J L,SU M,WANG L Y,et al. Exogenous hydrogen peroxide reversibly inhibits root gravitropism and induces hori- zontal curvature of primary root during grass pea germination [J]. Plant Physiology and Biochemistry,2012,53(4) :84- 93. 被引量:1
  • 4张军,孙树贵,王亮明,王秀娟,杨群会,陈新宏.孕穗期低温对冬小麦生理生化特性和产量的影响[J].西北植物学报,2013,33(11):2249-2256. 被引量:11
  • 5SAHIN CEVIK M, MOORE G A. Identification and expres- sion analysis of cold-regulated genes from the cold-hardy citrus relative Poncirus tri foliata (L.) Raf[J]. Plant Molecular Bi- ololy, 2006,62(1-2) : 83-97. 被引量:1
  • 6HUNG S H, WANG C C, IVANOV S V,et al. Repetition of hydrogen peroxide treatment induces a chilling tolerance con, parable to cold acclimation in mung bcan[J]. Journal of the American Society for Horticultural Science, 2007,132 ( 6 ) : 770-776. 被引量:1
  • 7GEETIKA SIRHINDI M K, BHARDWAJ R, KUMAR S, et al. Effect of exogenous H2O3 on antioxidant enzymes of Bras- sica juncea L. seedlings in relation to 24 epibrassinolide under chilling stress[J]. Indian Journal of Biochemistry and Bio- physics,2010,47(6) :378-382. 被引量:1
  • 8JIANG J L,SU M,CHEN Y R,et al. Correlation of drought resistance in grass pea (Lathyrus sativus IJ. ) with reactive ox- ygen species scavenging and osmotic adjustment[J]. Biologia, 2013,68(2):23-1 240. 被引量:1
  • 9. FAROOQ M,BASRA S M A,WAHID A,et al. Improving the drought tolerance in rice (Oryza sativa L. ) by exogenous ap- plication of salicylic acid[J]. Journal of Agronomy and Crop Science,2009,195(4) :237 246. 被引量:1
  • 10李玲主编..植物生理学模块实验指导[M].北京:科学出版社,2009:175.

二级参考文献234

共引文献97

同被引文献141

引证文献10

二级引证文献21

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈
新型冠状病毒肺炎防控与诊疗专栏