Objective To investigate the therapeutic effect of BMSCs transplant on AD model rats. Methods Rats were randomly divided into five groups,the normal group,model group,model control group,the treatment group,the treatment control group,n=10.After bilateral hippocampus injection Aβ23-35,The rat model of AD was established,BMSCs transplantation therapy in the treatment group.We performed gavage treatment with galantamine hydrobromide in the treatment control group. Results Behavioral experiments detected the average escape latency of rats and the first time across the original platform,Model group compared with normal group（P〈0.01）,treatment group and the treatment group compared with model group（P〈0.01）,compared with normal group（P〈0.05）,There were significant differences.Experimental Morphology HE staining showed that the model group granule cells and pyramidal cells have damage phenomenon,treatment group and control group obviously recovery.Immunohistochemistry showed that normal group and model control group showed more positive protein expression in the hippocampus,however the expression in the model group was significantly reduced.Treatment group and treatment group expression was significantly increased compared with the model group. Conclusion BMSCs transplantation treated AD which possible mechanism of action by adjusting the Wnt signaling pathway,affect learning and memory-related enzyme protein expression in the hippocampus play a role related.The expression of enzymes and proteins associated learning and memory in the hippocampus which play an important role.
China medicine and pharmacy