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广州市某医院周边鼠形动物肺炎克雷伯菌和铜绿假单胞菌的耐药性调查 预览 被引量:1

Investigation of antimicrobial resistance of Klebsiella pneumoniae and Pseudomonas aeruginosa isolates from rat-like animals around a hospital in Guangzhou
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摘要 目的:了解鼠形动物粪便标本中携带肺炎克雷伯菌与铜绿假单胞菌的耐药情况。方法2015年3月~10月,利用笼捕法于广州市某医院及其周边居民区捕获鼠形动物,分离其粪便中肺炎克雷伯菌与铜绿假单胞菌菌株,按照2014年CLSI标准进行药敏试验,利用SPSS 20.0软件分析数据。结果共捕获鼠形动物329只(包括205只臭鼩鼱、111只褐家鼠、5只黄胸鼠和8只小家鼠)。肺炎克雷伯菌与铜绿假单胞菌在鼠形动物体内检出率分别为78.4%和34.7%。臭鼩鼱携带的肺炎克雷伯菌对氨苄西林、头孢唑林、呋喃妥因、哌拉西林和头孢噻肟耐药性高(耐药率分别为100%、51.2%、44.2%、37.2%和23.3%);家鼠携带肺炎克雷伯菌耐药情况与鼩鼱相似。肺炎克雷伯菌的多重耐药率为40.9%,产ESBLs菌株检出率为10.7%。鼩鼱与家鼠携带的铜绿假单胞菌均对氨曲南耐药情况最严重(耐药率为12.4%和16.0%),其次为广谱青霉素类和喹诺酮类抗生素,对头孢菌素、氨基糖苷类和碳青霉烯类抗生素耐药率最低(耐药率均〈5%)。结论鼠形动物携带的肺炎克雷伯菌与铜绿假单胞菌的耐药谱与目前临床分离株的耐药谱相似,提示这两种细菌可能在鼠形动物与人之间有传播作用,值得进一步研究。 Objective To investigate antimicrobial resistance of Klebsiella pneumoniae and Pseudomonas aeruginosa isolates in fecal samples from rat-like animals. Methods Rat-like animals were captured using cages around a hospital and the neighboring residential area between March and October, 2015. K. pneumoniae and P. aeruginosa were isolated from the fecal samples of the captured animals. Antimicrobial susceptibility test was performed according to the guidelines of Clinical and Laboratory Standards Institute (2014). Results A total of 329 rat-like animals were captured, including 205 Suncus murinus, 111 Rattus norvegicus, 5 Rattus flavipectus and 8 Mus musculus. The positivity rates of K. pneumoniae and P. aeruginosa were 78.4%and 34.7%in the fecal samples from the captured animals, respectively. K. pneumoniae isolates from Suncus murinus showed a high resistance to ampicillin, cephazolin, nitrofurantoin, piperacillin and cefotaxime (with resistance rates of 100%, 51.2%, 44.2%, 37.2%, and 23.3%, respectively), and K. pneumoniae isolates from Rattus spp. showed a similar drug-resistance profile. The prevalence rates of multidrug resistance and ESBLs were 40.9%and 10.7%, respectively. P. aeruginosa from both Suncus murinus and Rattus spp. exhibited the highest resistance rates to aztreonam (12.4%and 16.0%, respectively), followed by penicillins and fluoroquinolones. P. aeruginosa isolates were susceptible to cephems, aminoglycosides and carbapenems (with resistance rates below 5%). Conclusion K. pneumoniae and P. aeruginosa isolated from rat-like animals showed drug-resistance profiles similar to those of the strains isolated from clinical patients, suggesting that the possible transmission of K. pneumoniae and P. aeruginosa between rat-like animals and human beings.
作者 钟雪珊 葛婧 陈少威 熊益权 郑雪燕 邱旻 霍舒婷 陈清 ZHONG Xueshan,GE Jing,CHEN Shaowei,XIONG Yiquan,ZHENG Xueyan,QIU Min,HUO Shuting,CHEN Qing(Department of Epidemiology, School of Public Health and Tropical Medicine, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, Guangzhou 510515, China)
出处 《南方医科大学学报》 CAS CSCD 北大核心 2016年第5期688-692,共5页 Journal of Southern Medical University
基金 国家自然科学基金(81373051)
关键词 家鼠 鼩鼱 铜绿假单胞菌 肺炎克雷伯菌 抗生素耐药性 Rattus spp. Suncus murinus Pseudomonas aeruginosa Klebsiella pneumonia antibiotic resistance
作者简介 钟雪珊,硕士研究生,E—mail:zhong_xueshan@163.com 通信作者:陈清,博士,教授,E-mail:qch.2009@163.com
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