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蛋白质指纹技术检测肺癌患者血栓形成的临床研究 被引量:1

Clinical research of detection of thrombosis in patients with lung cancer by protein fingerprinting
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摘要 目的探讨肺癌深静脉血栓患者血清蛋白质指纹的改变。方法选择肺癌患者18例,其中合并深静脉血栓8例,10例未合并深静脉血栓(对照组),应用表面增强激光解析电离化蛋白质.飞行时间质谱(SELDI-TOF-MS)技术对两组血清在相同质荷比(M/Z)的蛋白质含量进行分析,得出含量差异有统计学意义的蛋白质峰。结果对照组与肺癌合并深静脉血栓组指纹丰度相比M/Z为5911、1216、4187、1019、4293的5个蛋白质峰差异有统计学意义(43.81±7.74、6.37±5.02、2.97+0.35、35.96±12.10、9.65±4.37与15.35±12.69、2,06±0.37、4.67±1.35、15.94±6.47、14.65±8.80,t值分别为5.334、4.800、2.981、4.639、4.596,P均〈0.05),与对照组相比,深静脉血栓组下调的蛋白质峰M/Z为5911、1019、1216,上调的蛋白质峰M/Z为4293、4187。结论在肺癌患者血清中SELDI图谱M/Z为5911、4293、4187、1019、1216的SELDI蛋白质指纹可认为是肺癌患者合并血栓的特异性标记。 Objective To investigate the changes of serum protein fingerprint in patients with lung cancer with deep venous thrombosis. Methods Eighteen case patients with lung cancer were selected, including 8 case of lung cancers with thrombosis and 10 cases of lung cancers with no thrombosis. Surface enhanced laser desorption ionization protein-time of flight mass spectrometry (SELDI-TOF-MS) was used to analyze serum protein content of two groups in the same mass to charge ratios ( M/Z), then drew the protein peaks that content difference was statistically significant. Results The M/Z of lung" cancer with thrombus group and control group were 5911,1216,4187,1019,4293, the protein peaks had significant differences between the two group( 43. 81 ±7.74,6. 37±5.02,2. 97±0. 35,35.96±12. 10,9. 65 ± 4. 37; 15.35 ± 12. 69,2. 06± 0. 37,4. 67 ± 1.35, 15.94±6.47,14. 65±8. 80; t = 5. 334,4. 800,2. 981,4. 639,4. 596; P〈 0. 05 ). Compared with the control group, the decrease of protein peak M/Z were 5911,1019,1216,and the increase of protein peak M/Z were 4293,4187 in the deep venous thrombosis group. Conclusion In the serum of patients with tumors SELDI profiles M/Z are 5911,4293,4187,1019, 1216 of SELDI protein fingerprinting can be considered in patients with thrombosis of tumor specific markers.
作者 马瑜慧 Ma Yuhui ,Pei Yi ,Ji Lina ,Du Yang. (Internal Medicine Department ,Shanxi Academy of Medical Sciences, Shanxi Dayi Hospital, Taiyuan 030032, China)
出处 《中国综合临床》 2016年第5期437-439,共3页 Clinical Medicine of China
关键词 深静脉血栓 肺癌 蛋白质指纹 Deep venous thrombosis Lung cancer Protein fingerprint
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