Objective： Application for prenatal testing of fetal chromesome aneuploidies. Methods： Pregnant women in 562 cases with Detection of fetal free DNA in peripheral blood in hospital, from June 2013 to October 2015, are all single birth, and Gestational week is 14-26＋5weeks, which are divided into Old age group, High risk group, Critical risk group and other groups, according to the type.In order to verity the result, the amniotic cavity of those pregnant women should be punctured, their amniotic cells should be cultivated and their chromosomal karyotype should be analgzed. Rusults： The DNA of 562 samples were detected, It showed that 13 cases were chromosome abnormality, among which 12 cases received prenatal diagnoses and 1 case didn＇ t.12 cases received chromosomal diagnosis, among which 7 cases showed chromosome abnormalities and 5 cases were normal. The coincidence rate is 58.3% （7/12） , the coincidence rate is 99.1% （557/562） and the false positive rate is 0.89% （5/562） , and false negative cases haven＇t been found. Conclusion： Prenatal testing of fetal chromesome aneuploidies has High sensitivity and No trauma, Can be used as an auxiliary method of traditional prenatal diagnosis method. The sensitivity of this technalogy is hith for chromosomes 21 and 18, but it has limitations to test chromosone 13 and sex chromosomes.The false positive rate is high, which has bad effects on the patients＇ psychology.
Chinese Journal of Birth Health ＆ Heredity