目的探讨治疗期肺结核患者外周血T 细胞活性与相关细胞因子IL-2、IFN-γ变化的相关性及其临床意义.方法随机选取我院呼吸科2014 年1 月 ~2015年1 月期间内住院和门诊肺结核患者为研究对象,并选取40例健康人群为对照组.分离肺结核病患者和健康人群外周血单个核细胞,体外加PPD刺激培养后,用 MTT法测定 T淋巴细胞的增殖;并采用酶联免疫吸附 （ELISA） 法测定培养上清中IL-2、IFN-γ的水平.结果肺结核患者治疗前淋巴细胞活性和培养上清液中IL-2水平和IFN-γ水平均明显低于健康人对照组（P〈0.05）,而经过免疫治疗后3个月和6个月,细胞活性和细胞因子水平呈梯度变化,治疗6个月的细胞活性和IL-2、IFN-γ的水平与治疗前相比均有显著差异（P〈0.05）.结论肺结核患者在不同治疗阶段外周血的细胞免疫功能不断变化,监测机体细胞免疫状态,不仅能够指导临床用药,促进肺结核病患者的全面痊愈,而且能够建立稳定的外周血免疫细胞检测平台,为肺结核免疫疗法的发展完善提供参考依据.
ObjectiveTo investigate the correlation between T cells viability and serum IL-2 and IFN-γ in the peripheral blood of patients with pulmonary tuberculosis and its clinical significance. MethodsRandomly selected the patients with pulmonary tuberculosis from January 2014 to January 2o15 in our hospital department of respiration as the study object, and selected 40 cases of healthy people as control group. Mononuclear cells in the blood of patients with pulmonary tuberculosis and the healthy ones were separated. MTT assay was used to measure T cells proliferation, and then the serum levels of IL-2 and IFN-γ were measured by enzyme-linked immunosorbent assay （ELISA）. ResultsThe cell viability and serum IL-2 levels and IFN-γ levels of the patients with pulmonary tuberculosis were significantly lower than those in healthy controls （P〈0.05）, and the cell activity and the levels of cytokines gradient changed after 3 months and 6 months immunotherapy. There were significant differences in cell viability and the levels of IL-2 and IFN-γ between the 6 months of treatment and prior treatment （P〈0.05）. ConclusionThe cellular immune function of patients with pulmonary tuberculosis changes in different stages of treatment, monitoring the immune status of the body, not only can guide clinical medication, promoting the comprehensive recovery of patients with pulmonary tuberculosis, but also can establish a stable peripheral blood immune cell detection platform, providing a reference for the development of immune therapy.
Journal of Traditional Chinese Medicine University of Hunan
peripheral blood T cells