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RP-HPLC法测定防风通圣丸中栀子苷、芍药苷、黄芩苷、大黄素的含量 预览 被引量:3

Contents of Jasminoidin,Peoniflorin,Baicalin and Emodin in FangFeng TongSheng Pills Determined by RP-HPLC Method
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摘要 目的:建立RP-HPLC同时测定防风通圣丸中栀子苷、芍药苷、黄芩苷和大黄素含量的方法。方法:采用高效液相色谱(RP-HPLC)梯度洗脱,在不同波长下测定含量。色谱柱:Zorbax Eclipse XDB-C18键合硅胶色谱柱(4.6 mm×150 mm,5μm);柱温为室温;流动相为乙腈-2%磷酸水溶液,采用梯度洗脱;流速1.0 m L/min;检测波长分别为240 nm(栀子苷、芍药苷),275 nm(黄芩苷、大黄素)。结果:栀子苷、芍药苷、黄芩苷和大黄素的进样量分别在0.056~0.56 g(r=0.999 7),0.103 5~1.029 7 g(r=0.999 2),0.207 4~3.128g(r=0.999 3),0.04~0.423g(r=0.999 6)范围内线性关系良好,平均加样回收率分别为99.14%,99.65%,99.03%,99.16%;RSD分别为1.08%,1.01%,0.85%,0.86%。结论:该方法简便、快速、灵敏、精密度高、重现性良好、结果准确,可用于同时测定防风通圣丸中栀子苷、芍药苷、黄芩苷和大黄素的含量。 Objective:To establish the determination methods for the contents of jasminoidin,peoniflorin,baicalin and emodin in FangFeng TongSheng pills simultaneously by RP-HPLC.Methods:The contents were determined under different wavelengths by adopting RP-HPLC as gradient elution.Chromatographic column:Zorbax Eclipse XDB-C_(18) bonded silica gel column(4.6 mm×150 mm,5 μm);column temperature was room temperature;mobile phase:acetonitrile-2%phosphoric acid aqueous solution,gradient elution was adopted;flow rate 1.0mL/min;detection wavelengths were 240nm(jasminoidin and peoniflorin) and 275nm(baicalin and emodin) respectively.Results:Jasminoidin,peoniflorin,baicalin and emodin were in better linear relationship when their sample sizes were between 0.056 and 0.56g(r=0.999 7),0.103 5 and 1.029 7g(r=0.999 2),0.207 4 and 3.128 g(r=0.999 3),0.04 and0.423 g(r=0.999 6),average recovery rates were 99.14%,99.65%,99.03%and 99.16%;RSD was 1.08%,1.01%,0.85%and 0.86%respectively.Conclusion:The method,simple,rapid,sensitive,reproducible and of high precision and accurate results,could be used for content determination of jasminoidin,peoniflorin,baicalin and emodin in FangFeng TongSheng pills simultaneously.
作者 杨小军 方博文 丁永辉 YANG Xiaojun1, FANG Bowen1, DING Yonghui2(1 Chemical Engineering Institute of Northwest University for Nationalities, Lanzhou 730124, China; 2 Gansu Food and Drug Administration)
出处 《西部中医药》 2016年第9期27-34,共8页 Gansu Journal of Traditional Chinese Medicine
基金 国家自然科学基金项目(编号21402153)
关键词 RP-HPLC 防风通圣丸 栀子苷 芍药苷 黄芩苷 大黄素 含量测定 RP-HPLC FangFeng TongSheng pills jasminoidin peoniflorin baicalin emodin content determination
作者简介 作者简介:杨小军(1982-),男,硕士学位,讲师。研究方向:药物分析。
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