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UFLC法同时测定龙胆中3种环烯醚萜苷的含量 被引量:5

Simultaneous Determination of Three Iridoid Glycosides in Radix Gentianae by UFLC
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摘要 目的采用超快速液相色谱(UFLC)法同时测定龙胆中龙胆苦苷、獐芽菜苦苷和獐芽菜苷的含量。方法采用Shim-Pack XR-ODS柱(75 mm×3.0 mm,2.2μm),流动相为甲醇-0.1%磷酸水溶液(梯度洗脱),检测波长为240 nm,流速为0.5 m L·min-1,柱温为40℃。结果龙胆苦苷、獐芽菜苦苷和獐芽菜苷进样量分别在1.0~20μg(r=0.9998)、1.0~20μg(r=0.9995)、0.12~2.4μg(r=0.9996)范围内与峰面积呈良好的线性关系,平均加样回收率分别为97.3%、99.3%和98.3%(n=3),RSD均小于1.0%。结论 UFLC法简便、快速、准确、重现性好,可用于龙胆药材中环烯醚萜苷类成分的质量控制研究。 Objective To develop an ultra-fast liquid chromatography(UFLC)method for simultaneous determination of gentiopicroside,swertiamarin and sweroside in Radix Gentianae. Methods Chromatographic separation was carried out on a Shim-Pack XR-ODS column(75 mm×3.0 mm,2.2 μm)with a mobile phase consisting of acetonitrile and water(containing 0.1 % phosphoric acid)by gradient elution at a flow rate of 0.5 m L·min-1. The detecting wavelength was set at 240 nm and the column temperature was maintained at 40 ℃. Results The calibration curves of gentiopicroside,swertiamarin and sweroside showed good linear relationship in the range of 1.0-20 μg(r=0.9998),1.0-20 μg(r=0.9995)and 0.12-2.4 μg(r=0.9996)respectively,and the average recovery(n=9)of three components was 97.3 %, 99.3 % and 98.3 % respectively, and the relative standard deviation(RSD) was less than 1.0 %.Conclusion The developed UFLC method is simple, rapid, accurate and repeatable, which is suitable for the quality control of gentiopicroside,swertiamarin and sweroside in Radix Gentianae.
作者 关皎 朱鹤云 郝乘仪 郭淑英 冯波 GUAN Jiao, ZHU Heyun, HAO Chengyi, GUO Shuying, FENG Bo(College of Pharmacy, Jilin Medical University, Changchun 132013 Jilin, China)
出处 《中药新药与临床药理》 CAS CSCD 北大核心 2017年第1期81-84,共4页 Traditional Chinese Drug Research & Clinical Pharmacology
基金 吉林省卫生厅科研课题(2013Z008)
关键词 龙胆 龙胆苦苷 獐芽菜苦苷 獐芽菜苷 超快速液相色谱法 Radix Gentianae gentiopicroside swertiamarin sweroside ultra-fast liquid chromatography
作者简介 关皎,女,博士,讲师,研究方向:中药质量控制及药代动力学。Email:rainbowguanjiao@sina.com。 通信作者:冯波,男,博士,教授,研究方向:中药新药研发。Email:fengb02@sina.com。
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