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阿霉素对肾上腺皮质癌细胞系SW-13克隆特性的影响 预览

Effect of adriamycin on the characteristics of colony derived from human adrenal cortical carcinoma cells SW-13
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摘要 目的:研究阿霉素在体干预对肾上腺皮质癌系SW-13克隆特性的影响。方法:通过建立阿霉素干预裸鼠连续传代SW-13细胞系皮下移植瘤模型,将空白组及实验组细胞进行单细胞克隆行成试验,观察单个细胞生长曲线,并计算克隆形成率及3种克隆(全克隆、部分克隆和旁克隆)的百分比,并进行Hoechst33342试验检测细胞的耐药性。结果:SW-13细胞系经过阿霉素干预裸鼠连续传代移植瘤模型后,克隆形成率较空白对照组增加(P〈0.05);全克隆的百分比高于空白对照组(P〈0.05);经Hoeehst33342试验发现经阿霉素干预肾上腺皮质癌细胞荧光减弱。结论:阿霉素在体干预提高肾上腺皮质癌细胞系SW-13的克隆形成能力及全克隆形成能力,并提高其耐药性。 Objective To explore the effect of adriamycin on the characteristics of colony derived from human adrenal cortical carcinoma cells (ACC) SW-13. Methods Treatment with Adriamycin (ADM) was used in BALB/c-nude mouse tumor xenograft model established using the ACC cell line SW-13. The characteristic of colony was assessed for the formation rates, the percentage of three colony types and growth curve of single cell. Hoechst33342 dyeing test was used to test drug resistance. Results The Single-cell colony formation rate of experimental group were significantly higher than control group (P 〈 0.05), and the holoclone percentage of experimental group were significantly higher than control group (P 〈 0.05). In the Hoechst33342 dyeing test, the fluorescence intensity of control was higher than experimental group. Conclusion The treatment of ADM in vivo is beneficial for the colony formations of ACC cell and the formations rate of holoclone, and can improve the ability of drug resistance of ACC cell SW-13.
作者 曾文清 陈小舟 黄振兴 杨海燕 邝晓聪 罗佐杰 ZENG Wenqing, CHEN Xiaozhou, HUANG Zhenxing, YANG Haiyan, KUANG Xiaochong, LUO Zuojie,(The Second Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310005, China)
出处 《实用医学杂志》 CAS 北大核心 2017年第3期343-346,共4页 The Journal of Practical Medicine
基金 国家自然科学基金项目(编号:81060220) 广西教育厅项目(编号:12MS061) 浙江中医药大学校级课题(编号:2014ZY17)
关键词 肾上腺皮质肿瘤 克隆 全克隆 阿霉素 BALB/c-nu裸鼠 Adrenal cortical carcinoma Colony Holoclone Adriamycin BALB/c-nude mouse
作者简介 通信作者:罗佐杰 E-mail:Zluo888@163.com.cn
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