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虎耳草炭疽病病原菌鉴定 被引量:1

Identification of Colletotrichum species associated with Saxifraga stolonifera anthracnose
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摘要 【目的】从虎耳草炭疽病病斑分离病原菌,并对病原菌进行鉴定。【方法】采用柯赫氏法则对从病斑组织块分离的病原菌进行验证,通过形态学结合多基因分子系统学方法对病原菌进行鉴定。【结果】从虎耳草炭疽病病斑分离到2株菌株(菌株号:LPSU 20120244、LPSU 20120251)。2株菌株孢子均无色,无隔,直、圆柱状,菌株LPSU 20120244孢子(11-25)μm×(5-9)μm,菌株LPSU 20120251孢子(15-25)μm×(5-7)μm。多基因分子系统树中,2株菌株分别与喀斯特炭疽菌(Colletotrichum karstii Y.L.Yang,Zuo Y.Liu,K.D.Hyde&L.Cai)和江西炭疽菌(C.jiangxiense F.Liu&L.Cai)模式菌株聚为一支,支持率均为100%。【结论】形态学及多基因分子系统学分析表明菌株LPSU 20120244为喀斯特炭疽菌,菌株LPSU 20120251为江西炭疽菌。 [Objective] The study aims to isolate and identify the pathogen from Saxifraga stolonifera anthracnose. [Methods] The pathogen were isolated by tissue associated anthracnose and confirmed through Koch's postulates, then the strains were identified through morphologic characteristics and multi-locus(ACT, CAL, CHS-1, GAPDH, ITS, TUB2) phylogeny analysis. [Results] Two strains, isolated from the leaves of S. stolonifera, were numbered as LPSU 20120244 and LPSU 20120251. Conidium of both two strains was hyaline, aseptate, straight, cylindrical. Conidium size of LPSU 20120244 was(11-25) μm×(5-9) μm, LPSU 20120251,(15-25) μm×(5-7) μm. Strain LPSU 20120244 and LPSU 20120251 was clustered with Colletotrichum karstii Y.L. Yang, Zuo Y. Liu, K.D. Hyde L. Cai and C. jiangxiense F. Liu L. Cai, respectively, and the bootstrap support of both two clades were 100%. [Conclusion] Based on morphologic characteristics and multi-locus phylogeny analysis, the two strains represent 2 Colletotrichum species, Colletotrichum karstii(strain LPSU 20120244) and C. jiangxiense(strain LPSU 20120251).
作者 杨友联 李树江 刘作易 YANG You-Lian1,LI Shu-Jiang1,2,LIU Zuo-Yi3(1. Department of Life Sciences, Liupanshui Normal University, Shuicheng, Guizhou 553004, China;2. Institute of Biology, Liupanshui Normal University, Shuicheng, Guizhou 553004, China; 3. Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550006, China)
出处 《微生物学通报》 CAS CSCD 北大核心 2017年第4期818-824,共7页 Microbiology
基金 科技部科技基础性工作专项项目(No.2014FY120100) 贵州省自然科学基金项目(No.黔科合J字[2012]2304) 六盘水市科技合作项目(No.52020-2012-01-09-05) 贵州省植物学重点支持学科(No.黔学位合字ZDXK[2014]24号)
关键词 炭疽菌属 多基因分子系统学 柯赫氏法则 Colletotrichum Cda. Multi-locus phylogeny Koch's postulates
作者简介 通讯作者:Tel:86-858-83761761;E-mail:gzliuzuoyi@163.com
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