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2008—2013年上海市售海产品中副溶血性弧菌污染的监测和毒力基因分析 预览 被引量:4

Vibro parahemolyticus contamination surveillance and virulence genes analysis on commercial marine products during 2008-2013 in Shanghai
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摘要 目的了解上海市市售海产品中副溶血性弧菌的污染水平和特征。方法 2008—2013年,采用GB/T 4789.7—2008《食品卫生微生物学检验副溶血性弧菌检验》的方法,对上海市批发市场、集贸市场、卖场超市、餐饮环节和网购环节等污染物监测点采集的市售海产品进行副溶血性弧菌污染的定量检测,对副溶血性弧菌食品分离株采用PCR方法进行毒力基因耐热直接溶血素(tdh)、耐热直接溶血素相关溶血素(trh)的检测。结果共监测市售海产品2 643件,在192件样品中检出副溶血性弧菌,总检出率为7.3%;其中2.6%(5/192)的菌株tdh或trh阳性,tdh和trh的携带率分别为2.1%(4/192)和0.5%(1/192)。不同监测月份、不同食品类别和不同采样地点的海产品,其副溶血性弧菌污染水平总体上存在差异(P〈0.05)。其中6—10月份海产品副溶血性弧菌检出率和样品平均浓度显著高于其他月份(P〈0.05);海产鱼类的副溶血性弧菌检出率(8.4%)和几何平均浓度(0.209 MPN/g)显著高于其他种类海产品(P〈0.05);批发市场、集贸市场和卖场超市采集的海产品副溶血性弧菌浓度显著高于其他场所(P〈0.05)。结论上海市售海产品中副溶血性弧菌在夏秋季节污染水平较高,部分食品分离株携带有tdh或trh基因,应继续开展海产品中副溶血性弧菌污染的风险监测和探索其环境影响因素,并针对副溶血性弧菌污染的高风险节点开展风险评估和管理。 Objective To investigate and characterize the contamination of Vibrio parahaemolyticus(V. parahaemolyticus) in commercial seafoods on Shanghai markets. Methods Based on the method of GB/T 4789. 7-2008 from "Microbiological Examination of Food Hygiene: Examination of V. parahaemolyticus",the contamination of V. parahaemolyticus in seafoods from different predetermined monitoring sites in Shanghai was quantitatively determined and the occurrence of virulence genesthermostable direct hemolysin(tdh) and TDH-related hemolysin(trh),in 192 seafoods separated V. parahaemolyticus strains were detected by PCR. Results A total of 2 643 seafood samples from wholesale markets,retail markets,supermarkets,catering industries and online shopping were collected from January to December in the years of 2008 to 2013. V. parahaemolyticus was found in 192 samples(7. 3%). Among 192 separated strains,2. 6%(5/192) that carried tdh or trh. 2. 1%(4/192) were positive for tdh,and 0. 5%(1/192) were positive for trh. The geometric mean densities of V. parahaemolyticus were different significantly in varied seafood species,sampling months,as well as locations(P〈0. 05). Especially,geometric mean densities of V. parahaemolyticus in June to October were significantly higher than those in other months(P〈0. 05). The detection rate(8. 4%) and geometric mean densities(0. 209 MPN/g) of V. parahaemolyticus in fishes were significantly higher than those in other seafood(P〈0. 05). The geometric mean densities of V. parahaemolyticus in seafoods from wholesale markets,retail markets,supermarkets were higher than those from other sampling resources(P〈0. 05). Conclusion The contamination of V. parahaemolyticus in seafoods during summer and fall is relatively higher from Shanghai markets. Some separated V. parahaemolyticus strains carry virulence genes tdh and trh. Thus the contamination of V. parahaemolyticus in seafood needs to be intensively monitored and environmental impact factors should
作者 田明胜 彭少杰 陈波 厉曙光 TIAN Ming-sheng, PENG Shao-jie, CHEN Bo, LI Shu-guang(School of Public Health, Fudan University, Shanghai 200032, China; General Enforcement Team of Shanghai Food and Drug Administration, Shanghai 200233, China)
出处 《上海预防医学》 CAS 2017年第6期435-439,共5页 Shanghai Journal of Preventive Medicine
关键词 海产品 副溶血性弧菌 监测 毒力基因 seafoods Vibrio parahaemolyticus contaminant monitoring virulence genes
作者简介 田明胜(1978-),男,主管医师,在读博士研究生
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