期刊文献+

Wnt3a蛋白对牙髓干细胞成骨向分化的影响

Effects of Wnt3a on osteogenic differentiation of dental pulp stem cells
分享 导出
摘要 目的探讨Wnt3a蛋白对牙髓干细胞(dentalpulpstemcells,DPSC)成骨向分化的影响,进一步说明Wnt信号通路在DPSC分化中的作用。方法将不同质量浓度『0(培养液组)、5、20、50及100vg/L1的Wnt3a蛋白作用于DPSC,于诱导培养的第7天检测其碱性磷酸酶(alkalinephosphatase,ALP)活性,对检测结果进行单因素方差分析;茜素红染色检测矿化结节形成情况;通过实时荧光定量PCR(quantitativereal.timePCR,qPCR)检测骨涎蛋白、I型胶原、Runt相关转录因子2(Runt—relatedtranscriptionfactor-2,RUNX2)及骨钙蛋白4种骨源性基因的表达,对结果数据采用独立样本t检验进行统计学分析。结果DPSC诱导7d后检测结果显示,Wnt3a蛋白可抑制ALP的活性,0、5、20、50及100μg/L组ALP的活性分别为1.076±0.203、0.828±0.118、0.505±0.044、0.499±0.038及0.483±0.060,Wnt3a蛋白质量浓度越高对ALP活性的抑制作用越强;qPCR检测显示,5txg/LWnt3a蛋白组骨钙蛋白基因阳性表达水平(0.092±0.005)显著低于培养液组(0.858±0.190)(P〈0.05);28d后茜素红染色结果显示,5μg/LWnt3a蛋白对DPSC无明显的诱导矿化现象。结论Wnt3a蛋白可抑制DPSC成骨向分化。 Objective To investigate the effect of Wnt3a on osteogenic differentiation of human dental pulp stem cells (DPSC). Methods DPSCs were subjected to different concentrations of Wnt3a (0, 5, 20, 50 and 100 μg/L) and at seven days after culture the alkaline phosphatase (ALP) activity was tested. Mineralized nodule formation was examined by alizarin red staining. Osteogenic-related gene expression of bone sialoprotein (BSP), osteocalcin (OCN), collagen type I (COL- I ), Runt-related transcription factor-2 (RUNX2) was examined by quantitative real-time PCR (qPCR). Results After seven days of induction by DPSC, Wnt3a protein could inhibit the ALP activity (concentration 0: 1.076±0.203, 5 μg/L: 0.828±0.118, 20 μg/L: 0.505±0.044, 50 μg/L: 0.499±0.038, 100 μg/L: 0.483±0.060). The expression of OCN in 5 μg/L Wnt3a group (0,092±0.005) was lower than that in culture medium (0.858±0.190)(P〈0.05). Alizarin red staining showed that 5 μg/L Wnt3a had no mineralization induction effect on DPSC. Conclusions Wnt3a could inhibit osteogenic differentiation of dental pulp stem cells.
作者 孙艳艳 胡伟平 刘宗响 王伟 Sun Yanyan, Hu Weiping, Liu Zongxiang, Wang Wei (1.Department of Prosthoclontics, Xuzhou Stomatology Hospital, Xazhou Jiangsu 221000, China ; 2.Department of Prosthodontics, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China ; 3.Department of Periodontics, Xuzhou Stomatology Hospital, Xuzhoa Jiangsu 221000, China )
出处 《中华口腔医学杂志》 CSCD 北大核心 2017年第7期427-431,共5页 Chinese Journal of Stomatology
关键词 WNT蛋白质类 牙髓干细胞 成骨向分化 Wnt proteins Dentalpulp stem cells Osteogenesis
作者简介 通信作者:王伟,Email:wwxzkq@163.com,电话:0516-85866037
  • 相关文献

参考文献2

二级参考文献40

  • 1何飞 ,谭颖徽 ,张纲 .人牙髓干细胞的体外培养和鉴定[J].华西口腔医学杂志,2005,23(1):75-78. 被引量:18
  • 2Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo[J]. Proc Natl Acad Sci USA, 2000, 97(25): 13625-13630. 被引量:1
  • 3Miura M, Gronthos S, Zhao MR, et al. SHED: stem cells from human exfoliated deciduous teeth[J]. Proc Nat Acad Sci USA, 2003,100(10): 5807-5812. 被引量:1
  • 4Wakitani S, Saito T, Caplan AT. Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine [ J ].Muscle Nerve, 1995, 18(12): 1417-1422. 被引量:1
  • 5Pittenger MF, Mackay AM, Beck SC, et al. Multilieage potential of adult human mesenchymal stem cells [J]. Science, 1999, 284(5411): 143-147. 被引量:1
  • 6Smith AG. Embryo-derived stem cells: of mice and men[J]. Annu Rev Cell Dev Biol,2001, 17(1): 435-462. 被引量:1
  • 7Bjornson CR, Rietze RL, Reynolds BA, et al. Turning brain into blood: a hematopoietic fate adopted by adult neural stem cells in vivo [J]. Science, 1999, 283(5401): 537-547. 被引量:1
  • 8Bouvier M, Joffre A, Magloire H. In vitro mineralization of a threedimensional collagen matrix by human dental pulp cells in the presence of chondroitin sulphate[J]. Arch Oral Biol, 1990,35(4) :301-307. 被引量:1
  • 9Imad A, Bottero MJ, Denato PD, et al. Human dentin production in vitro [J]. Exp Cell Res, 2000, 258(1): 33-41. 被引量:1
  • 10Tsukamoto Y, Fukutani S, Shin-Ike T, et al. Mineralized nodule formation by cultures of human dental pulp-derived fibroblasts[J]. Arch Oral Biol, 1992,37(12): 1045-1055. 被引量:1

共引文献17

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈