以紫霞黄栌（Cotinus coggygria var.‘Zixia’）为材料,组培紫霞黄栌外植体,获得最适宜紫霞黄栌的组培条件,得到一套紫霞黄栌苗木资源大规模生产的组织培养技术规范。最适宜的诱导分化培养基为MS基础培养基添加1.0 mg/L 6-BA和0.1 mg/L NAA,扦插苗、一年生枝条和二年生枝条作为外植体的诱导率分别达到96.7%、93.3%和73.3%。外植体材料在5～8月适宜采集。增殖培养最适宜条件为以改良MS培养基作为基础培养基,pH调至5.4,添加100μmol/L 6-BA、1μmol/L NAA、10μmol/L IAA并添加1 mg/L维生素C,评价得分4.23分,增殖系数最高达到8.97。生根培养使用MS改良培养基,添加0.3 mg/L IBA和0.2 mg/L NAA时生根率达到53%。移栽以消毒灭菌后的珍珠岩作为基质,温度控制为25℃,相对湿度保持在90%,移栽后组培苗成活率达到70%。使用该方法可大幅提高紫霞黄栌增殖数量。
Continus coggygria var. ‘Zixia＇ was used as the material to cultivate the explants of ‘Zixia＇ through tissue culture.Aim to obtain the optimum culture condition of ‘Zixia＇ and study the key technology of tissue culture and rapid propagation for ‘Zixia＇,in order to get a set of large-scale production of ‘Zixia＇ seedling tissue culture technical specifications. The optimum inducing differentiation medium was MS ＋1.0 mg/L 6-BA ＋0.1 mg/L NAA,the sprouting rate of cutting seedling,annual branches and biennial branches were 96.7%, 93.3% and 73.3% respectively. The suitable time for obtained explants were May to August. The optimum conditions for the proliferation culture were as follows ：us modified MS medium,the pH was adjusted to 5.4, add 100 μmol/L 6-BA,1 μmol/L NAA, 10 μmol/L IAA, and 1 mg/L VC. The score was 4.23 and the proliferation coefficient reached 8.97. In the rooting culture stage was use modified MS medium,add with 0.3 mg/L IBA and 0.2mg/L NAA, the rooting rate was 53%. During the transplanting stage, the perlite with sterile was used as the substrate,the temperature of greenhouse was controlled at 25 ℃,the relative humidity was kept at 90%,and the survival rate of plantlet was70%. The use of this method can greatly improve proliferation of Cotinus coggygria var. ‘Zixia＇.
Hubei Agricultural Sciences