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驼峰藤组织培养及快速繁殖 预览 被引量:2

Tissue Culture and Rapid Propagation of Merrillanthus hainanensis
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摘要 以驼峰藤(Merrillanthus hainanensis)带芽茎段为外植体,研究不同消毒方法及植物生长调节剂对其离体培养及植株再生的影响,建立了驼峰藤离体快繁技术体系。结果表明,带芽茎段的最佳消毒灭菌方法为75%乙醇浸泡20 s,0.1%HgCl_2浸泡10 min;腋芽诱导的最适宜培养基为MS+2.00 mg/L 6-BA+0.10mg/L NAA+0.10 mg/L GA_3,诱导率可达81.11%;适宜腋芽增殖的培养基为MS+3.50 mg/L 6-BA+0.15mg/L NAA,增殖系数可达6.24;最佳生根培养基为1/2MS+0.30 mg/L NAA,生根率为84.44%,该结果为驼峰藤的快繁提供了一条新途径。 In order to establish the tissue culture and rapid propagation system of Merrillanthus hainanensis,the effects of different disinfection method and plant growth regulators on plant regeneration in vitro were studied using stems with axillary buds as explants. The results showed that the better disinfection method for stems with axillary buds was to use 75% alcohol immersion 20 s,then use 0.1% HgCl_2 immersion 10 min;the fittest medium for germination of stem axillary bud was MS+2.00mg/L 6-BA +0.10 mg/L NAA +0.10 mg/L GA_3,with a 81.11% germination rate of axillary bud. The best medium for shoot multiplication was MS +3.50 mg/L 6-BA +0.15 mg/L NAA,the multiplication coefficient could reach 6.24. The best rooting medium was 1/2MS+0.30 mg/L NAA,with a 84.44% rooting rate. The research supplied a new way for rapid propagation of Merrillanthus hainanensis.
作者 侯俊 王彩云 张翔宇 阮培均 HOU Jun1, WANG Cai-yun2, ZHANG Xiang-yu2, RUAN Pei-jun2 (1.Poverty Alleviation and Development Office of Dafang County ,Bijie 551700,Guizhou,China; 2.Bijie Institute of Traditional Chinese Medicine,Bijie 551700,Guizhou,China)
出处 《湖北农业科学》 2017年第17期3345-3348,共4页 Hubei Agricultural Sciences
基金 国家“十二五”科技支撑计划项目(2015BAI05B03) 毕节市成果转化项目[毕科成字(2014)8号] 毕节市科技支撑计划项目[毕科合(2016)48号]
关键词 驼峰藤(Merrillanthus hainanensis) 带芽茎段 组织培养 快速繁殖 Merrillanthus hainanensis stems with axillary buds tissue culture rapid propagation
作者简介 侯俊(1987-),男,贵州普安人,助理农艺师,学士,主要从事植物资源工程研究工作,(电话)15772712527(电子信箱)406359837@qq.com 通信作者,阮培均,男,研究员,主要从事药用植物遗传与育种研究,(电子信箱)rpj3819@126.com。
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