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经典干细胞标志物CD34、PDGF-βR、VEGF-R2在早孕蜕膜组织中的表达研究 预览

Expression of Typical Stem Cell Marker CD34, PDGF-βR and VEGF-R2 in Human Early Pregnancy Decidua
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摘要 目的 研究经典干细胞标志物CD34、PDGF-βR、VEGF-R2在早孕6-8周蜕膜组织中的表达.方法 提取6-8周早孕期人工流产术蜕膜组织(n=16),消化后选用CD34、PDGF-βR、VEGF-R2单克隆抗体进行荧光染色,分别采用单染、双染及三染法,应用流式细胞仪技术分析上述经典干细胞标志物的表达及其相互关系.结果 6-8周早孕期蜕膜组织细胞中,(1)单染法证实CD34+细胞为(0.81±1.07)%,PDGF-βR+细胞为(9.94±7.36)%,VEGF-R2+细胞为(0.90±1.19)%;(2)双染法证实CD34+/VEGFR2-细胞(0.59±0.61)%,CD34+/VEGF-R2+细胞(0.16±0.10)%;(3)三染法证实CD34+/VEGFR2+/PDGF-βR+细胞(0.09±0.06)%,CD34+/VEGFR2-/PDGF-βR+细胞(0.15±0.13)%. 结论 早孕6-8周蜕膜组织中存在经典干细胞标志物CD34、PDGF-βR、VEGF-R2阳性表达的细胞,存在少量的双阳性表达及三阳性表达的细胞.早孕蜕膜组织中存在于细胞参与其发育过程. Objective To study the expression of CD34,PDGF-βR and VEGF-R2 in the human decidual tissue of 6-8 weeks gestation.Methods The decidua tissue collected following induced abortion during 6-8 weeks of gestation was digested to form a single cell suspension.The expression of CD34,PDGF-βR and VEGF-R2 monoclonal antibodies were analyzed by single,double,and triple staining method.Flow cytometry was used to analyze the expression of these markers and their correlation.Results (1) In the 6-8 weeks early pregnancy decidual tissue cells,the concentration of CD34 +cells was (0.81 ± 1.07)%,PDGF-[3R+ cells (9.94 ± 7.36)%,and VEGF-R2 + cells (0.90 ± 1.19)%.(2) The concentration of CD34 +/VEGFR2-cells was (0.59 ± 0.61) %,and CD34 + / VEGF-R2 + cells (0.16 ± 0.10) %.(3) The concentration of CD34 + / VEGF-R2 +/PDGF-[3R + cells was (0.09 ± 0.06) %,and CD34 + / VEGF-R2-/PDGF-βR + cells (0.15 ±0.13)%.Conclusion The expression of CD34,PDGF-βR and VEGF-R2 in the early pregnancy decidual tissue was found,and there were also small amount of double positive and triple positive expression of the cells.Existence of stem cells in decidual tissue in early pregnancy suggests that those cells may be involved in the development of the decidua.
作者 郎福地 郭丽群 孙欣 杨舒奇 孙敬霞 田海 黄明莉 LANG Fudi, GUO Liqun, SUN Xin, YANG Shuqi, SUN Jingxia, TIAN Hai, HUANG Mingli( Department of Gynecology and Obstetrics, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China)
出处 《中国生育健康杂志》 2017年第5期441-446,共6页 Chinese JOurnal of Reproductive Health
基金 国家自然科学基金青年科学基金项目(81401203) 黑龙江省自然科学基金(H201433) 哈尔滨市科学技术局科技创新人才基金(2013RFLYJ003)
关键词 早孕蜕膜组织 干细胞 流式细胞仪 CD34 PDGF-βR VEGF-R2 Deciduas of early pregnancy Stem cells Flow cytometry CD34 PDGF -βR VEGF-R2
作者简介 通讯作者:黄明莉(doctor_hml@163.com)
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