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鳖甲炮制前后抗肝纤维化活性部位的SDS-PAGE凝胶电泳比较研究

Comparison on SDS-PAGE Gel Electrophoresis of Anti-liver Fibrosis Components in Pre- and Post-pro- cessed Trionyx Sinensis Carapace
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摘要 目的:探讨鳖甲炮制前后抗肝纤维化活性部位的差异,为鳖甲药材的临床用药提供依据。方法:运用SDS-PAGE技术,对生鳖甲与醋鳖甲活性部位进行分析比较;采用MTT比色法测定生鳖甲与醋鳖甲对HSC-T6增殖的影响。结果:炮制后的醋鳖甲所含成分增多,一级谱带相对分子质量降低,导致小分子肽类物质产生;生鳖甲和醋鳖甲均具有抗肝纤维化的作用,但醋鳖甲的药效优于生鳖甲。结论:鳖甲炮制前后活性部位明显差异,为临床选择用药及进一步活性成分研究提供依据。 Objective: To study the difference in anti-hepatic fiborsis components between pre- and post-processed Trionyx Sinensis Carapace to guide the clinical application of Trionyx Sinensis Carapace. Methods: SDS-PAGE gel electrophoresis was used to compare the constituents in pre- and post-processed Trionyx Sinensis Carapace, and the inhibitory effect on the proliferation of HSC-T6 was determined by MTT. Results: The processed Trionyx Sinensis Carapace had much more components than the crude Trionyx Sinensis Carapace, and the relative molecular mass in the first-level band decreased resulting in the generation of micromolecular polypeptides. Both pre- and post-processed Trionyx Sinensis Carapace had anti-hepatic fibrosis, while the vinegar-processed Trionyx Sinensis was more ef- fective. Conclusion: The difference in the active components in pre- and post-processed Trionyx Sinensis Carapace is obvious, which provides foundation for the clinical application and further researches.
作者 高建蓉 胡祖良 施静妮 唐尹萍 刘焱文 胡春玲 Gao Jianrong1 , Hu Zuliang1 , Shi Jingni2 , Tang Yinping2 , Liu Yanwen2 , Hu Chunling2 ( 1. Zhejiang Quhua Hospital, Zhejiang Quzhou 324004, China ; 2. Key Laboratory of Chinese Medicine Chemistry and Chinese Herbal Compound of Hubei Province, Hubei University of Traditional Chinese Medicine)
出处 《中国药师》 CAS 2017年第9期1543-1544,1563共3页 China Pharmacist
基金 浙江省自然科学基金项目(编号:LY14H280001) 湖北省教育厅重点项目(编号:D20162004)
关键词 鳖甲 十二烷基磺酸钠-聚丙烯酰胺凝胶电泳 活性部位 Trionyx sinensis carapace SDS-PAGE gel electrophoresis Active components
作者简介 通讯作者:胡春玲Tel:13971227875E—mail:1054681196@qq.com
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