期刊文献+

江苏省2013年-2015年风疹病毒基因特征分析

Genetic characteristics analysis of rubella virus strains isolated in Jiangsu from 2013 to 2015
分享 导出
摘要 目的分析江苏省2013年-2015年风疹病毒(RV)流行毒株的基因特征。方法采集该期间疑似风疹病例咽拭子标本,于Vero-Slam细胞上进行病毒分离,采用逆转录PCR(RT-PCR)方法对分离到的7株RV E1基因739个核苷酸片段进行扩增,对该PCR产物进行序列测定和分析,并与世界卫生组织(WHO)RV各基因型参考株进行分子流行病学研究。结果江苏省3株RV分离株属于1E基因型,核苷酸同源性为99.2%-100.0%,氨基酸同源性为99.6%-100.0%。4株RV分离株属于2B基因型,核苷酸同源性为98.9%-100.0%,氨基酸同源性为100.0%。血凝抑制位点和中和位点等抗原表位未发生变异,1E基因型风疹毒株在E1蛋白的aa338(Leu338→Phe338)、aa357(Leu357→Val357)发生变异。结论江苏省2013年-2015年风疹病毒的优势基因型为1E和2B基因型,与目前我国其他省份流行的风疹病毒基因型一致,E1基因氨基酸序列高度保守。 Objective To analyze the genetic characteristics of rubella virus( RV) strains isolated in Jiangsu province from2013 to 2015. Methods Rubella virus strains were isolated on Vero-Slam cells from the throat swabs samples of the suspected patients. The fragments containing 739 nucleotides of E1 genes from 7 rubella virus strains were amplified by RT-PCR method,the PCR products were directly sequenced and analyzed and WHO rubella virus reference strains were conducted for epidemiologic study. Results 3 isolated strains were closely related to reference strains of 1 E genotypes,the homology of nucleotide and amino acid were within 99. 2%-100. 0% and 99. 6%-100. 0%,respectively. Four isolated strains were closely related to reference strains of 2 B genotypes,the homology of nucleotide and amino acid were within 98. 9%-100. 0% and100. 0%,respectively. No variation was detected at hemagglutination inhition and neutralization sites. Two amino acid mutation from Leu to Phe in the aa338 amino acid and from Leu to Val in the aa357 amino acid of E1 protein was identified,respectively.Conclusion Genotype 1 E and 2 B of rubella virus were the predominant genotypes in Jiangsu province during 2013-2015,which was similar to the other provinces in China. The amino acid sequence of E1 protein was highly conservative.
作者 邓秀英 陆培善 郭宏雄 冷红英 吴昀 胡莹 DENG Xiu- ylng, LU Pei -shah, GUO Hong- xiong, LENG Hong- ying, WU Yun, HU Ying (Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, JiangSu 210009, China)
出处 《中国卫生检验杂志》 CAS 2017年第20期2995-2998,3001共5页 Chinses Journal of Health Laboratory Technology
关键词 风疹病毒 基因型 基因特征 Rubella virus Genotype Genetic characterization
作者简介 邓秀英(1983-),女,硕士,主管技师,主要从事计划免疫相关疾病的实验室检测工作。 通讯作者:胡莹,E—mail:26137089@qq.com
  • 相关文献

参考文献4

二级参考文献61

共引文献35

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈