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PELP1在炎性牙周膜细胞中的表达及与细胞增殖和成骨分化的相关性研究

Expression of PELP1 in inflammatory periodontal ligament cells and its correlation with cell proliferation and ostegenic differentiation
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摘要 目的:探讨PELP1与牙周膜细胞骨向分化的相关性。方法:通过对正常人牙周膜细胞与炎症人牙周膜细胞的分离、培养、鉴定,采用RT-PCR、免疫细胞化学、免疫荧光化学及Western blot技术,分别从基因和蛋白水平检测PELP1在两组细胞中的表达及其在细胞增殖和骨向分化过程中的表达变化。结果:在H-hPDLCs和I-hPDLCs中均存在PELP1 mRNA及蛋白的表达。PELP1参与调节hPDLCs的生长与增殖,在H-hPDLCs中与细胞增殖呈正相关,在I-hPDLCs中与细胞增殖呈负相关。PELP1的表达水平在两组hPDLCs骨向分化中亦表现出不同的变化趋势,在H-hPDLCs骨向诱导分化后升高,在I-hPDLCs骨向诱导分化后降低。结论:PELP1在炎症人牙周膜细胞中表达并参与调节细胞的生长增殖及骨向分化。 Objective:To investigate the relationship between PELP1 and bone differentiation in periodontal ligament cells. Methods:Human periodontal ligament cells( hPDLCs) were cultured and identified by immunocytochemistry. RT-PCR and Western blot were performed to detect PELP1 expression on proliferation and osteogenic differentiation. Results:The results showed that PELP1 was expressed in both H-hPDLFCs and Ⅰ-hPDLFCs. Compared with PELP1 expression in H-hPDLF-Cs,the PELP1 level of Ⅰ-hPDLFCs decreased with culture time. During osteogenic induction,the PELP1 expression in Ⅰ-hP-DLFCs was decreased with culture time,and similar with the expression of the osteogenic marker osteocalcin. Conclusion:PELP1 expressed in hPDLCs and played a role in PDLCs' proliferation and osteogenic differentiation.
作者 王婧 祝强 李学盛 庞恋苏 王燕一 WANG Jing, ZHU Qiang, LI XUE-sheng, PANG Lian-su, WANG YAN-yi (1. Department of Stomatology, Hainan Branch, General Hospital of the Chinese People's Liberation Army, Hainan Sanya 572000, China ;2. Department of Urology, General Hospital of the Chinese People' s Liberation Army ,Beijing 100853, China.)
出处 《临床口腔医学杂志》 2017年第11期643-647,共5页 Journal of Clinical Stomatology
基金 全军医学科技青年培育项目(15QNP076) 海南省自然基金项目(20168360)
关键词 牙周膜细胞 PELP1 成骨分化 增殖 Osteogenic induction Proliferation
作者简介 通讯作者:王燕一,Tel:0898-37331154 E-mail:wyy301@hotmail.com
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