Objective： To establish a multiple myeloma specimen bank applied for molecular biological researches and to explore the methods of specimen collection,transportation,storage,quality control and the management of specimen bank.Methods： Bone marrow and blood samples were collected from multiple myeloma patients,plasma cell sorting were operated after the separation of mononuclear cells from bone marrow specimens. The plasma cells were divided into 2 parts,one was added with proper amount of TRIzol and then kept in -80 ℃ refrigerator for subsequent RNA extraction,the other was added with proper amount of calf serum cell frozen liquid and then kept in -80 ℃ refrigerator for subsequent cryopreservation of DNA extraction after numbered respectively. Serum and plasma were separated from peripheral blood,specimens of serum and plasma were then stored at -80 ℃ refrigerator after registration. Meantime,the myeloma specimen information management system was established,managed and maintained by specially-assigned persons and continuous modification and improvement in the process of use as to facilitate the rapid collection,management,query of the effective samples and clinical data.Results： A total of 244 portions plasma cells,564 portions of serum,and 1005 portions of plasma were collected,clinical characters were documented. Conclusion： A multiple myeloma specimen bank have been established initially,which can provide quality samples and related clinical information for molecular biological research on multiple myeloma.
Journal of Experimental Hematology